Hypertonicity and/or acidosis induce marked rheological changes under hypoxic conditions in sickle trait red blood cells

被引：3

作者：

Ellsworth, Patrick ^{[1
,2
]}

Pawlinski, Izabela J. ^{[1
]}

Sielaty, Rachel ^{[1
]}

Ilich, Anton ^{[1
]}

Prokopenko, Yuriy ^{[1
]}

Moonla, Chatphatai ^{[1
,3
,4
,5
]}

Monroe, Dougald M. ^{[1
,2
]}

Pawlinski, Rafal ^{[1
,2
]}

Key, Nigel S. ^{[1
,2
]}

机构：

[1] Univ North Carolina Chapel Hill, UNC Blood Res Ctr, Chapel Hill, NC USA

[2] Univ North Carolina Chapel Hill, Dept Med, Div Hematol, CB 7305,116 Manning Dr, Chapel Hill, NC 27599 USA

[3] Chulalongkorn Univ, Dept Med, Bangkok, Thailand

[4] Chulalongkorn Univ, Fac Med, Ctr Excellence Translat Hematol, Bangkok, Thailand

[5] King Chulalongkorn Mem Hosp, Bangkok, Thailand

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 2024年 / 205卷 / 04期

基金：

美国国家卫生研究院;

关键词：

hypoxia; red cell; rheology; sickle cell trait;

D O I：

10.1111/bjh.19669

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Deformability and sickling of red blood cells (RBCs) from individuals with sickle cell trait (SCT) was evaluated under harsh biophysical conditions that mimic certain vascular beds in vivo. RBC deformability in osmotic-gradient ektacytometry was decreased in HbAS (SCT) compared to HbAA (wild-type) RBCs at supraphysiological osmolalities. RBC deformability was also measured by oxygen-gradient ektacytometry. Whereas RBC sickling was not observed under isotonic and neutral pH conditions, hypertonicity and acidosis alone or in combination induced reversible sickling of SCT RBC. These data suggest that hyperosmolality and/or acidosis enhance hypoxia-induced sickling of SCT RBC.

引用

页码：1565 / 1569

页数：5

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