Production and molecular weight variation of poly-γ-glutamic acid using a recombinant Bacillus subtilis with various Pgs-component ratios

被引：1

作者：

Sawada, Kazuhisa ^{[1
,2
,3
]}

Hagihara, Hiroshi ^{[1
]}

Takimura, Yasushi ^{[1
]}

Kataoka, Masakazu ^{[1
,2
,4
]}

机构：

[1] Kao Corp, Global R&D Biol Sci Res, Haga, Tochigi, Japan

[2] Shinshu Univ, Dept Biomed Engn, Grad Sch, Wakasato, Nagano, Japan

[3] Kao Corp, Technol Dev Ctr, SCM, 2-1-3 Bunka,Sumida Ward, Tokyo, Japan

[4] Grad Sch Shinshu Univ, Dept Biomed Engn, 4-17-1 Wakasato, Nagano, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2024年

关键词：

poly-gamma-glutamic acid; Bacillus subtilis; PgsBCA component; recombinant production; D/L ratio; ESCHERICHIA-COLI; GENE; SEQUENCE; TRANSFORMATION; EXPRESSION; NETWORK;

D O I：

10.1093/bbb/zbae093

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly-gamma-glutamic acid (PGA) has been of interest as a sustainable biopolymer in industrial applications. PGA biosynthesis in Bacillus subtilis is catalyzed by a transmembrane protein complex comprising PgsB, PgsC, and PgsA. To determine the Pgs component responsible for PGA overproduction, we constructed recombinants in which the promoter of the host-derived pgs gene was replaced with another host-derived gene promoter. These recombinants were then transformed using high-copy-number plasmids with various pgs-gene combinations to enhance Pgs component in different ratios. Subsequently, PGA production was investigated in batch cultures with l-glutamate supplemented medium. The recombinant strain enhanced with pgsB alone significantly overproduced PGA (maximum production 35.8 g/L) than either the pgsC- or pgsA-enhanced strain. The molecular weight of the PGA produced with the pgsB-enhanced strain was also greater than that for the pgsC- or pgsA-enhanced strain (approximately 10-fold). Hence, PgsB enhancement alone contributes to PGA overproduction with increased molecular weight.

引用

页码：1217 / 1224

页数：8

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