Electrostatic interaction-mediated 1:1 complexes for high-contrast mitochondrial-targeted phosphorescence bioimaging

被引:2
作者
Cheng, Jianshuo [1 ]
Sun, Hao [1 ]
Zhou, Lulu [1 ]
Baryshnikov, Glib V. [2 ]
Liu, Mouwei [1 ]
Shen, Shen [1 ]
Agren, Hans [3 ]
Zhu, Liangliang [1 ]
机构
[1] Fudan Univ, Dept Macromol Sci, State Key Lab Mol Engn Polymers, Shanghai 200438, Peoples R China
[2] Linkoping Univ, Dept Sci & Technol, Lab Organ Elect, SE-60174 Norrkoping, Sweden
[3] Uppsala Univ, Dept Phys & Astron, SE-75120 Uppsala, Sweden
基金
瑞典研究理事会; 中国国家自然科学基金;
关键词
room-temperature phosphorescence; mitochondrial targeting; bioimaging; complex; electrostatic interaction; ROOM-TEMPERATURE PHOSPHORESCENCE; ORGANIC PHOSPHORESCENCE; PERSULFURATED BENZENE; AGGREGATE; TUMOR;
D O I
10.1007/s11426-024-2158-7
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Organelle-targeted imaging can provide information on cellular functions and intracellular interactions, being significant for disease diagnosis. The use of room-temperature phosphorescence (RTP) in organelle-targeted imaging can fully utilize its unique characteristics of long wavelength and deep penetration. However, this technology has long been plagued by insufficient probe targeting and limited luminous intensity. In this work, we prepared a series of complexes composed of multicationic persulfurated arenes and biomacromolecules via electrostatic interactions in 1:1 stoichiometry for high-contrast mitochondrial-targeted RTP imaging. Such an electrostatic interaction design effectively prevented the self-aggregation of the probes, which is not conducive to mitochondrial targeting. Simultaneously, it suppressed the non-radiative decay to the maximum extent, enabling the probes to exhibit strong RTP signals both in aqueous solution and at the cellular level. Furthermore, the biomacromolecules can serve as carriers for an electrostatic interaction transfer of the persulfurated arenes to mitochondria. This leads to high mitochondrial targeting Pearson's correlation coefficients of the probes and high-contrast RTP imaging effects, as well as the independence of the co-incubated probe concentration. These results provide new insights for the development of targeted imaging technologies.
引用
收藏
页码:3406 / 3413
页数:8
相关论文
共 50 条
[1]   Red AIE-Active Fluorescent Probes with Tunable Organelle-Specific Targeting [J].
Alam, Parvej ;
He, Wei ;
Leung, Nelson L. C. ;
Ma, Chao ;
Kwok, Ryan T. K. ;
Lam, Jacky W. Y. ;
Sung, Herman H. Y. ;
Williams, Ian D. ;
Wong, Kam Sing ;
Tang, Ben Zhong .
ADVANCED FUNCTIONAL MATERIALS, 2020, 30 (10)
[2]   A persulfurated benzene molecule exhibits outstanding phosphorescence in rigid environments: from computational study to organic nanocrystals and OLED applications [J].
Bergamini, Giacomo ;
Fermi, Andrea ;
Botta, Chiara ;
Giovanella, Umberto ;
Di Motta, Simone ;
Negri, Fabrizia ;
Peresutti, Romain ;
Gingras, Marc ;
Ceroni, Paola .
JOURNAL OF MATERIALS CHEMISTRY C, 2013, 1 (15) :2717-2724
[3]   Assembly strategies of organic-based imaging agents for fluorescence and photoacoustic bioimaging applications [J].
Cheng, Hong-Bo ;
Li, Yuanyuan ;
Tang, Ben Zhong ;
Yoon, Juyoung .
CHEMICAL SOCIETY REVIEWS, 2020, 49 (01) :21-31
[4]   Targeted Solvatochromic Fluorescent Probes for Imaging Lipid Order in Organelles under Oxidative and Mechanical Stress [J].
Danylchuk, Dmytro, I ;
Jouard, Pierre-Henri ;
Klymchenko, Andrey S. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2021, 143 (02) :912-924
[5]   Recent Advances of Pure Organic Room Temperature Phosphorescence Based on Functional Polymers [J].
Ding, Bingbing ;
Ma, Xiang ;
Tian, He .
ACCOUNTS OF MATERIALS RESEARCH, 2023, 4 (10) :827-838
[6]   Afterglow bio-applications by utilizing triplet excited states of organic materials [J].
Fan, Yuanyuan ;
Li, Qianqian ;
Li, Zhen .
SCIENCE CHINA-CHEMISTRY, 2023, 66 (11) :2930-2940
[7]   Recent advances in multicolor organic room-temperature phosphorescence [J].
Gan, Nan ;
Zou, Xin ;
Zhang, Yushan ;
Gu, Long ;
An, Zhongfu .
APPLIED PHYSICS REVIEWS, 2023, 10 (02)
[8]   Activated supramolecular nano-agents: From diagnosis to imaging-guided tumor treatment [J].
Han, Jingjing ;
Li, Haidong ;
Yoon, Juyoung .
NANO TODAY, 2022, 43
[9]   Engineering of Reversible NIR-II Redox-Responsive Fluorescent Probes for Imaging of Inflammation In Vivo [J].
He, Long ;
He, Lin-Hui ;
Xu, Shuai ;
Ren, Tian-Bing ;
Zhang, Xing-Xing ;
Qin, Zuo-Jia ;
Zhang, Xiao-Bing ;
Yuan, Lin .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2022, 61 (46)
[10]   Recent Advances in Materials with Room-Temperature Phosphorescence: Photophysics for Triplet Exciton Stabilization [J].
Hirata, Shuzo .
ADVANCED OPTICAL MATERIALS, 2017, 5 (17)