Potential Involvement of MnCYP710A11 in Botrytis cinerea Resistance in Arabidopsis thaliana and Morus notabilis

被引:0
作者
An, Hui [1 ,2 ]
Wang, Donghao [3 ]
Yu, Lin [3 ]
Wu, Hongshun [3 ]
Qin, Yue [1 ,2 ]
Zhang, Shihao [1 ,2 ]
Ji, Xianling [3 ]
Xin, Youchao [3 ]
Li, Xiaodong [1 ,2 ]
机构
[1] Hechi Univ, Guangxi Key Lab Sericulture Ecol & Appl Intelligen, Hechi 546300, Peoples R China
[2] Hechi Univ, Guangxi Collaborat Innovat Ctr Modern Sericulture, Sch Chem & Bioengn, Hechi 546300, Peoples R China
[3] Shandong Agr Univ, Coll Forestry, Tai An 271018, Peoples R China
关键词
cytochrome P450; B; cinerea; mulberry; STEROL C-22 DESATURASE; CYTOCHROME-P450; SYNTHASE; FRUIT; BIOSYNTHESIS; METABOLISM; PATHOGENS; ENCODES; DEFENSE; FAMILY;
D O I
10.3390/genes15070853
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Cytochrome P450 (CYP) is a crucial oxidoreductase enzyme that plays a significant role in plant defense mechanisms. In this study, a specific cytochrome P450 gene (MnCYP710A11) was discovered in mulberry (Morus notabilis). Bioinformatic analysis and expression pattern analysis were conducted to elucidate the involvement of MnCYP710A11 in combating Botrytis cinerea infection. After the infection of B. cinerea, there was a notable increase in the expression of MnCYP710A11. MnCYP710A11 is overexpressed in Arabidopsis and mulberry and strongly reacts to B. cinerea. The overexpression of the MnCYP710A11 gene in Arabidopsis and mulberry led to a substantial enhancement in resistance against B. cinerea, elevated catalase (CAT) activity, increased proline content, and reduced malondialdehyde (MDA) levels. At the same time, H2O2 and O-2- levels in MnCYP710A11 transgenic Arabidopsis were decreased, which reduced the damage of ROS accumulation to plants. Furthermore, our research indicates the potential involvement of MnCYP710A11 in B. cinerea resistance through the modulation of other resistance-related genes. These findings establish a crucial foundation for gaining deeper insights into the role of cytochrome P450 in mulberry plants.
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页数:12
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