Structural characteristics and biological activity of a water-soluble polysaccharide HDCP-2 from Camellia sinensis

被引：8

作者：

Sun, Qiaoxu ^{[1
]}

Du, Jiao ^{[1
]}

Wang, Zhen ^{[1
]}

Li, Xinyue ^{[1
]}

Fu, Ranze ^{[1
]}

Liu, Hui ^{[1
]}

Xu, Na ^{[2
]}

Zhu, Guoqi ^{[1
]}

Wang, Bin ^{[1
,3
]}

机构：

[1] Anhui Univ Chinese Med, Coll Chinese Med, Key Lab Xinan Med, Minist Educ,Funct Act & Resource Utilizat Edible &, Hefei 230038, Peoples R China

[2] Anhui Agr Univ, State Key Lab Tea Plant Biol & Utilizat, Hefei 230036, Anhui, Peoples R China

[3] Anhui Acad Chinese Med, Inst Pharmaceut Chem, Hefei 230038, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 277卷

关键词：

Structural characteristics; Camellia Sinensis (L.) O. Kuntze; Large-leaf yellow tea; Tea polysaccharides; Anti-inflammatory; IMMUNOSTIMULATORY ACTIVITY; TEA POLYSACCHARIDE; GREEN TEA; EXTRACTION; PURIFICATION; ANTIOXIDANT;

D O I：

10.1016/j.ijbiomac.2024.134437

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Large-leaf Yellow tea (LYT) is a traditional beverage from Camellia Sinensis (L.) O. Kuntze in China and has unusual health-regulating functions. This investigation explored the structural characteristics of a polysaccharide extracted from LYT, which possesses anti-inflammatory activity. The polysaccharide HDCP-2, obtained through ethanol fractional precipitation and then DEAE-52 anion exchange column, followed by DPPH radical scavenging screening, exhibited a yield of 0.19 %. The HPGPC method indicated that the molecular weight of HDCP-2 is approximately 2.9 x 104 4 Da. Analysis of the monosaccharide composition revealed that HDCP-2 consisted of mannose, glucose, xylose, and galacturonic acid, and their molar ratio is approximately 0.4:0.5:1.2:0.7. The structure motif of HDCP-2 was probed carefully through methylation analysis, FT-IR, and NMR analysis, which identified the presence of f3-D-Xylp(1-*, D-Xyl p (1-*,-*2, 4)-f3-D-Xylp(1-*, D-Xyl p (1-*,-*3)-f3-D-Manp(1-*, D-Man p (1-*, a-D-Glcp(1-* D-Glc p (1-* and-*2, 4)-a-D- D- GalAp(1-* p (1-* linkages. A CCK-8 kit assay was employed to evaluate the anti-inflammatory action of HDCP-2. These results demonstrated that HDCP-2 could inhibit the migration and proliferation of the MH7A cells and reduce NO production in an inflammatory model induced by TNF-a. The abundant presence of xylose accounted for 39% of the LYT polysaccharide structure, and its distinctive linking mode (-*2, 4)-f3-D-Xylp(1-*) D-Xyl p (1-*) appears to be the primary contributing factor to its anti-inflammatory effect.

引用

页数：10

共 48 条

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