Identification of angiogenesis-related genes and molecular subtypes for psoriasis based on random forest algorithm

被引:0
作者
Zhang, Meng-Jie [1 ]
Xue, Ting-Ting [1 ]
Fei, Xiao-Ya [2 ]
Zhang, Ying [2 ]
Luo, Ying [1 ,3 ]
Ru, Yi [1 ,3 ]
Jiang, Jing-Si [2 ]
Song, Jian-Kun [2 ]
Kuai, Le [1 ,3 ]
Luo, Yue [2 ]
Wang, Rui-Ping [2 ]
Li, Bin [2 ,3 ]
机构
[1] Shanghai Univ Tradit Chinese Med, Yueyang Hosp Integrated Tradit Chinese & Western M, Dept Dermatol, Shanghai 200437, Peoples R China
[2] Tongji Univ, Shanghai Skin Dis Hosp, Inst Dermatol, Sch Med, Shanghai 200443, Peoples R China
[3] Shanghai Acad Tradit Chinese Med, Inst Dermatol, Shanghai 201203, Peoples R China
基金
中国国家自然科学基金; 上海市科技启明星计划;
关键词
angiogenesis; psoriasis; molecular subtype; machine learning; SURVIVAL; EXPRESSION; CANCER; VEGF; IL-8; BIOLOGICS; ENDOMUCIN; ANGPTL4; PROMOTE; RISK;
D O I
10.1093/cei/uxae052
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Psoriasis is a chronic immune-mediated recurrent skin disease causing systemic damage. Increased angiogenesis has been reported to participate in the progression of psoriasis. However, angiogenesis-related genes (ARGs) in psoriasis have not been systematically elucidated. Therefore, we aim to identify potential biomarkers and subtypes using two algorithmsr. Transcriptome sequencing data of patients with psoriasis were obtained, in which differentially expressed genes were assessed by principal component analysis. A diagnostic model was developed using random forest algorithm and validated by receiver operating characteristic (ROC) curves. Subsequently, we performed consensus clustering to calculate angiogenesis-associated molecular subtypes of psoriasis. Additionally, a correlation analysis was conducted between ARGs and immune cell infiltration. Finally, validation of potential ARG genes was performed by quantitative real-time PCR (qRT-PCR). We identified 29 differentially expressed ARGs, including 13 increased and 16 decreased. Ten ARGs, CXCL8, ANG, EGF, HTATIP2, ANGPTL4, TNFSF12, RHOB, PML, FOXO4, and EMCN were subsequently sifted by the diagnostic model based on a random forest algorithm. Analysis of the ROC curve (area under the curve [AUC] = 1.0) indicated high diagnostic performance in internal validation. The correlation analysis suggested that CXCL8 has a high positive correlation with neutrophil (R =0.8, P < 0.0001) and interleukins pathway (R = 0.79, P < 0.0001). Furthermore, two ARG-mediated subtypes were obtained, indicating potential heterogeneity. Finally, the qRT-PCR demonstrated that the mRNA expression levels of CXCL8 and ANGPTL4 were elevated in psoriasis patients, with a reduced expression of EMCN observed. The current paper indicated potential ARG-related biomarkers of psoriasis, including CXCL8, ANGPTL4, and EMCN, with two molecular subtypes.
引用
收藏
页码:199 / 212
页数:14
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