Melaleuca alternifolia essential oil in a natural product-based formulation: Antimicrobial and healing effects in Staphylococcus aureus-infected wounds

被引:1
作者
Alves, Lully Gabrielly Silva [1 ,2 ]
Nova, Beatriz Gomes Vila [1 ]
Assuncao, Raissa Guara [1 ,3 ]
da Silva, Leticia Cely Tavares [1 ]
Sa, Gabrielle Chave [1 ]
dos Santos Silva, Lucas [1 ]
Silva, Marcos Andrade [1 ]
de Santana, Amanda Vitoria Sevidanes [1 ]
de Jesus, Thays Rodrigues [2 ]
Lucena, Flavia Ritchelle Coutinho [2 ]
dos Santos da Silva, Marcos Aurelio [1 ]
da Silva, Luis Claudio Nascimento [1 ]
Serra, Izabel Cristina Portela Bogea [2 ]
Abreu, Afonso Gomes [1 ,3 ]
机构
[1] CEUMA Univ, Microbial Pathogen Lab, Sao Luis, MA, Brazil
[2] CEUMA Univ, Pharm Lab, Sao Luis, MA, Brazil
[3] Univ Fed Maranhao, Biol & Hlth Sci Ctr, Hlth Sci Grad Program, Sao Luis, Brazil
关键词
Staphylococcus aureus; Melaleuca essential oil; Cream; Healing; Antimicrobial; TEA TREE OIL; MANAGEMENT;
D O I
10.1016/j.ejpb.2024.114416
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Owing to its exposed nature, the skin can be injured by various factors, including by Staphylococcus aureus, which inhabits its innate microbiota. Treatment of infected wounds presents an important challenge, making it imperative to develop new treatment options. Plant-derived formulations, such as those containing Melaleuca alternifolia essential oil (MaEO), are used for wound treatment because of their healing, anti-inflammatory, and antimicrobial properties. This study presents a cream containing 2% MaEO (2% CMa) and evaluates its effects in an S. aureus-infected wound murine model. The 2% CMa was subjected to quality control testing and pH and analysis of density, organoleptic characteristics, and microbiological effects. The quality control parameters all revealed the good stability of the 2% CMa. The formulation strongly reduced the S. aureus ATCC 6538 colonyforming unit (CFU) count in an ex vivo porcine skin model. In the murine model, daily topical application of 2% CMa reduced the severity and size of S. aureus-infected wounds and the bacterial load. These effects may be due to the presence of terpinen-4-ol, which exhibits anti-inflammatory activity. Based on these findings, the formulation exhibits good quality and safety. We suggest the topical application of this formulation, which exhibited an antimicrobial effect, as an interesting treatment strategy for wound healing.
引用
收藏
页数:9
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