Retinal organoids in disease modeling and drug discovery: Opportunities and challenges

被引：8

作者：

Chakrabarty, Koushik ^{[1
]}

Nayak, Divyani ^{[1
]}

Debnath, Jayasree ^{[1
]}

Das, Debashish ^{[2
]}

Shetty, Rohit ^{[3
]}

Ghosh, Arkasubhra ^{[1
]}

机构：

[1] Narayana Nethralaya Fdn, GROW Res Lab, Bangalore 560099, Karnataka, India

[2] Narayana Nethralaya, Narayana Nethralaya Fdn, Stem Cell Res Lab, GROW Lab, Bangalore, Karnataka, India

[3] Narayana Nethralaya Eye Hosp, Dept Cornea & Refract Surg, Bangalore, Karnataka, India

来源：

SURVEY OF OPHTHALMOLOGY | 2024年 / 69卷 / 02期

关键词：

Neural retina; IPSCs; Retinal degeneration; Retinal pigmented epithelium; Differentiation; Animal models; PLURIPOTENT STEM-CELLS; CONE PHOTORECEPTORS; MACULAR DEGENERATION; PIGMENT EPITHELIUM; EPIGENETIC MEMORY; SELF-FORMATION; DIFFERENTIATION; REVEALS; CULTURES; MOUSE;

D O I：

10.1016/j.survophthal.2023.09.003

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Diseases leading to retinal cell loss can cause severe visual impairment and blindness. The lack of effective therapies to address retinal cell loss and the absence of intrinsic regeneration in the human retina leads to an irreversible pathological condition. Progress in recent years in the generation of human three-dimensional retinal organoids from pluripotent stem cells makes it possible to recreate the cytoarchitecture and associated cell-cell interactions of the human retina in remarkable detail. These human three-dimensional retinal organoid systems made of distinct retinal cell types and possessing contextual physiological responses allow the study of human retina development and retinal disease pathology in a way animal model and two-dimensional cell cultures were unable to achieve. We describe the derivation of retinal organoids from human pluripotent stem cells and their application for modeling retinal disease pathologies, while outlining the opportunities and challenges for its application in academia and industry.

引用

页码：179 / 189

页数：11

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