Direct fluorescence and spectrophotometric-based detection of azithromycin using fluorescein isothiocyanate: Method development and comparative analysis

被引:2
作者
Hasaneen, Noha [1 ]
Pulicharla, Rama [1 ]
Brar, Satinder Kaur [1 ]
Rezai, Pouya [2 ]
机构
[1] York Univ, Lassonde Sch Engn, Dept Civil Engn, Toronto, ON M3J 1P3, Canada
[2] York Univ, Lassonde Sch Engn, Dept Mech Engn, Toronto, ON M3J 1P3, Canada
来源
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING | 2024年 / 12卷 / 03期
关键词
Azithromycin; Fluorescein isothiocyanate; Detection; Fluorescence; Spectrophotometric analysis; Contaminants; Urine; LOD; LOQ; PHARMACEUTICAL FORMULATIONS; MACROLIDE ANTIBIOTICS; HPLC; CLARITHROMYCIN; VALIDATION; ASSAY;
D O I
10.1016/j.jece.2024.112803
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
New methods for the analysis of azithromycin (AZM) have been devised, employing direct fluorescence and spectrophotometric techniques. This research focused on the development and validation of these methods, especially based on the formation of an ion-pair complex with fluorescein isothiocyanate (FITC). The resulting complex enhanced the fluorescence intensity at a wavelength of 520 nm and the absorbance at 480 nm. The impact of different solvent compositions was investigated, with methanol and a 1:1 mixture of methanol and Milli-Q proving to be the most sensitive. The optimal FITC concentration was determined as 50 mu g/ml, and the molar ratio of the AZM-FITC ion-pair complex was determined to be 1:1. Notably, an instantaneous increase in fluorescence intensity was observed, eliminating the need for incubation. The developed methods were validated for sensitivity, accuracy, and precision, with the fluorescence-based method demonstrating superior sensitivity and precision compared to the spectrophotometric method. Under optimum conditions, the fluorescence-based method achieved a linear range of 0-31.25 mu g/ml with a limit of detection (LOD) of 0.41 mu g/ml and a limit of quantification (LOQ) of 1.23 mu g/ml., In contrast, the spectrophotometric method had an LOD of 0.82 mu g/ml and an LOQ of 2.49 mu g/ml. Additionally, the fluorescence-based method exhibited a narrower recovery range in artificial urine samples, suggesting higher precision in complex matrices. Overall, these methods offer rapid and sensitive AZM analysis with potential applications in diverse matrices such as biological samples post-extraction.
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页数:9
相关论文
共 55 条
[1]  
Abdelmageed OH, 2006, B PHARM SCI, V29, P388
[2]  
Abdullah JH., 2014, J CHEM PHARM RES, V6, P202
[3]   Charge-transfer complexation of TCNE with azithromycin, the antibiotic used worldwide to treat the coronavirus disease (COVID-19). Part IV: A comparison between solid and liquid interactions [J].
Adam, Abdel Majid A. ;
Refat, Moamen S. ;
Altalhi, Tariq A. ;
Alsuhaibani, Khaled Saleh .
JOURNAL OF MOLECULAR LIQUIDS, 2021, 340
[4]   Charge-transfer chemistry of azithromycin, the antibiotic used worldwide to treat the coronavirus disease (COVID-19). Part III: A green protocol for facile synthesis of complexes with TCNQ, DDQ, and TFQ acceptors [J].
Adam, Abdel Majid A. ;
Saad, Hosam A. ;
Alsuhaibani, Amnah M. ;
Refat, Moamen S. ;
Hegab, Mohamed S. .
JOURNAL OF MOLECULAR LIQUIDS, 2021, 335
[5]   Charge-transfer chemistry of azithromycin, the antibiotic used worldwide to treat the coronavirus disease (COVID-19). Part II: Complexation with several π-acceptors (PA, CLA, CHL) [J].
Adam, Abdel Majid A. ;
Saad, Hosam A. ;
Alsuhaibani, Amnah M. ;
Refat, Moamen S. ;
Hegab, Mohamed S. .
JOURNAL OF MOLECULAR LIQUIDS, 2021, 325
[6]   Charge-transfer chemistry of azithromycin, the antibiotic used worldwide to treat the coronavirus disease (COVID-19). Part I: Complexation with iodine in different solvents [J].
Adam, Abdel Majid A. ;
Saad, Hosam A. ;
Alsuhaibani, Amnah M. ;
Refat, Moamen S. ;
Hegab, Mohamed S. .
JOURNAL OF MOLECULAR LIQUIDS, 2021, 325
[7]  
Ajima U., 2015, Der Pharm. Chem., V7, P160
[8]   Analysis of Azithromycin and Its Related Compounds by RP-HPLC with UV Detection [J].
Al-Rimawi, Fuad ;
Kharoaf, Maher .
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2010, 48 (02) :86-90
[9]   Fluorescence Determination of Azithromycin in Pharmaceutical Formulations by Using the Synchronous Scanning Approach After its Acid Derivatization [J].
Almeida, Vanessa G. K. ;
Braga, Victor S. M. ;
Pacheco, Wagner F. ;
Cassella, Ricardo J. .
JOURNAL OF FLUORESCENCE, 2013, 23 (01) :31-39
[10]   High performance liquid chromatographic determination of azithromycin in serum using fluorescence detection and its application in human pharmacokinetic studies [J].
Bahrami, BG ;
Mirzaeei, S ;
Kiani, A .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 820 (02) :277-281