1H, 15N and 13C resonance assignments of eggcase silk protein 3

被引：1

作者：

Yu, Shuixin ^{[1
]}

Qin, Ruiqi ^{[1
]}

Yuan, Wensu ^{[1
]}

Lin, Zhi ^{[1
]}

机构：

[1] Tianjin Univ, Sch Life Sci, Tianjin 300072, Peoples R China

来源：

BIOMOLECULAR NMR ASSIGNMENTS | 2024年 / 18卷 / 02期

基金：

中国国家自然科学基金;

关键词：

NMR; Assignment; ECP3; Silk; SPIDER; SEQUENCE; STRATEGY;

D O I：

10.1007/s12104-024-10192-4

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Spider silk is a high-performance biomaterial known for its outstanding combination of strength and flexibility. Among the six distinct types of spider silk, eggcase silk stands out as it is exclusively produced from the tubuliform gland, playing a specialized role in offspring protection. In the spider species Latrodectus hesperus, eggcase silk is spun from a large spidroin complex, including the major silk component tubuliform spidroin 1 (TuSp1) and at least six different minor silk components. One of these minor components is eggcase protein 3 (ECP3), a small silk protein of 11.8 kDa that lacks the typical spidroin architecture. ECP3 shows very limited homology to all known spidroins. In this study, we report nearly complete backbone and side-chain resonance assignments of ECP3 as a basis for studying the structural mechanisms involved in eggcase silk formation.

引用

页码：227 / 232

页数：6

共 16 条

[1] NMRPIPE - A MULTIDIMENSIONAL SPECTRAL PROCESSING SYSTEM BASED ON UNIX PIPES [J].

DELAGLIO, F ;

GRZESIEK, S ;

VUISTER, GW ;

ZHU, G ;

PFEIFER, J ;

BAX, A .

JOURNAL OF BIOMOLECULAR NMR, 1995, 6 (03) :277-293

[2] Critical role of minor eggcase silk component in promoting spidroin chain alignment and strong fiber formation [J].

Fan, Tiantian ;

Qin, Ruiqi ;

Zhang, Yan ;

Wang, Jingxia ;

Fan, Jing-Song ;

Bai, Xiangli ;

Yuan, Wensu ;

Huang, Weidong ;

Shi, Shuo ;

Su, Xun-Cheng ;

Yang, Daiwen ;

Lin, Zhi .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2021, 118 (38)

[3] 1H, 15N and 13C resonance assignments of a repetitive domain of tubuliform spidroin 2 [J].

Fan, Tiantian ;

Zhang, Yan ;

Fan, Jing-Song ;

Yuan, Wensu ;

Lin, Zhi .

BIOMOLECULAR NMR ASSIGNMENTS, 2021, 15 (02) :475-477

[4] Modular evolution of egg case silk genes across orb-weaving spider superfamilies [J].

Garb, JE ;

Hayashi, CY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (32) :11379-11384

[5]

Gosline JM, 1999, J EXP BIOL, V202, P3295

[6] Spider egg case core fibers: Trimeric complexes assembled from TuSp1, ECP-1, and ECP-2 [J].

Hu, XY ;

Kohler, K ;

Falick, AM ;

Moore, AMF ;

Jones, PR ;

Vierra, C .

BIOCHEMISTRY, 2006, 45 (11) :3506-3516

[7] Characterization and expression of a cDNA encoding a tubuliform silk protein of the golden web spider Nephila antipodiana [J].

Huang, W. ;

Lin, Z. ;

Sin, Y. M. ;

Li, D. ;

Gong, Z. ;

Yang, D. .

BIOCHIMIE, 2006, 88 (07) :849-858

[8] From EST to novel spider silk gene identification for production of spidroin-based biomaterials [J].

Huang, Weidong ;

Zhang, Yan ;

Chen, Yifan ;

Wang, Yin ;

Yuan, Wensu ;

Zhang, Ning ;

Lam, Toong Jin ;

Gong, Zhiyuan ;

Yang, Daiwen ;

Lin, Zhi .

SCIENTIFIC REPORTS, 2017, 7

[9]

Johnson Bruce A, 2004, Methods Mol Biol, V278, P313

[10] Sequence-specific assignment of aromatic resonances of uniformly 13C,15N-labeled proteins by using 13C- and 15N-edited NOESY spectra [J].

Lin, Z ;

Xu, YQ ;

Yang, S ;

Yang, DW .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (12) :1960-1963

← 1 2 →