A highly efficient coumarin-based turn-on fluorescent probe for specific and sensitive detection of exogenous and endogenous Nitroxyl in vivo and in vitro

被引：4

作者：

Zhou, Xin ^{[1
]}

Xu, Tongtong ^{[1
]}

Du, Wenhao ^{[1
]}

Liang, Yueyin ^{[1
]}

Gong, Shuai ^{[1
]}

Meng, Zhiyuan ^{[1
]}

Xu, Xu ^{[1
]}

Wang, Shifa ^{[1
]}

Wang, Zhonglong ^{[1
]}

机构：

[1] Nanjing Forestry Univ, Coll Chem Engn, Jiangsu Coinnovat Ctr Efficient Proc & Utilizat Fo, Int Innovat Ctr Forest Chem & Mat, Nanjing 210037, Peoples R China

来源：

JOURNAL OF MOLECULAR STRUCTURE | 2025年 / 1319卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; Nitroxyl detection; Coumarin; Living cells; Zebrafish; NITRIC-OXIDE; HNO;

D O I：

10.1016/j.molstruc.2024.139412

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Nitroxyl (HNO) is documented to be closely concerned with diverse pathological and physiological processes within living organisms. This research showcased the creation of a smart fluorescent turn-on probe, CCA-HNO, which is based on coumarin. It is designed to specifically detect HNO in the presence of metal ions, anions, biothiols, and reactive oxygen species (ROS). The probe CCA-HNO exhibited low detection limit of 34 nM and wide pH applicable range of 2-12 in the detection process of HNO. In view of its low cytotoxicity and admirable cell permeability, the probe CCA-HNO was utilized for high-contrast imaging of exogenous and endogenous HNO fluctuations in living cells. Moreover, the probe CCA-HNO also can effectively realize the real-time visualization of exogenous and endogenous HNO in living zebrafish. We anticipated that this finding can provide an excellent method for knowing the biological function of HNO in relevant pathological processes.

引用

页数：9

共 49 条

[1] A Chemiluminescent Probe for HNO Quantification and Real-Time Monitoring in Living Cells [J].