Complete genome sequence of an umbravirus from white snakeroot (Ageratina altissima)

被引:0
作者
Aboughanem-Sabanadzovic, Nina [1 ]
Sabanadzovic, Sead [1 ,2 ]
机构
[1] Mississippi State Univ, Inst Genom Biocomp & Biotechnol, Mississippi State, MS 39762 USA
[2] Mississippi State Univ, Dept Agr Sci & Plant Protect, Mississippi State, MS 39762 USA
基金
美国食品与农业研究所;
关键词
VIRUS;
D O I
10.1007/s00705-024-06125-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Complete genome sequencing of a virus from a white snakeroot plant (Ageratina altissima (L.) King & H. Rob.) collected in the Great Smoky Mountains National Park, USA, revealed a quadricistronic organization resembling that of umbraviruses. ORFs 1 and 2 are putatively translated via a -1 ribosomal frameshift mechanism as a single polypeptide with a role in viral replication, whereas the 3 '-proximal and extensively overlapping ORFs 3 and 4 code for proteins involved in long distance trafficing and cell-to-cell movement within the host. Sequence comparisons and phylogenetic analysis strongly suggested that this virus is a previously undescribed member of the genus Umbravirus (family Tombusviridae), for which the name "white snakeroot virus A" (WSVA) is proposed. In addition, we identified and initiated characterization of its possible helper virus, a putative new member of the genus Luteovirus.
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页数:4
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共 21 条
[1]   trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses [J].
Capella-Gutierrez, Salvador ;
Silla-Martinez, Jose M. ;
Gabaldon, Toni .
BIOINFORMATICS, 2009, 25 (15) :1972-1973
[2]  
Couch JF, 1927, J AGRIC RES, V35, P0547
[3]   Optimized approaches for the sequence determination of double-stranded RNA templates [J].
Darissa, Omar ;
Willingmann, Peter ;
Adam, Guenter .
JOURNAL OF VIROLOGICAL METHODS, 2010, 169 (02) :397-403
[4]   THE CHIMERIC NATURE OF THE GENOME OF PEA ENATION MOSAIC-VIRUS - THE INDEPENDENT REPLICATION OF RNA-2 [J].
DEMLER, SA ;
RUCKER, DG ;
DEZOETEN, GA .
JOURNAL OF GENERAL VIROLOGY, 1993, 74 :1-14
[5]   A RANDOM-PCR METHOD (RPCR) TO CONSTRUCT WHOLE CDNA LIBRARY FROM LOW AMOUNTS OF RNA [J].
FROUSSARD, P .
NUCLEIC ACIDS RESEARCH, 1992, 20 (11) :2900-2900
[6]  
Kalyaanamoorthy S, 2017, NAT METHODS, V14, P587, DOI [10.1038/NMETH.4285, 10.1038/nmeth.4285]
[7]   MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability [J].
Katoh, Kazutaka ;
Standley, Daron M. .
MOLECULAR BIOLOGY AND EVOLUTION, 2013, 30 (04) :772-780
[8]   IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies [J].
Lam-Tung Nguyen ;
Schmidt, Heiko A. ;
von Haeseler, Arndt ;
Bui Quang Minh .
MOLECULAR BIOLOGY AND EVOLUTION, 2015, 32 (01) :268-274
[9]   Red clover-associated luteovirus - a newly classifiable member of the genus Luteovirus with an enamo-like P5 protein [J].
Lenz, Ondrej ;
Sarkisova, Tatiana ;
Koloniuk, Igor ;
Franova, Jana ;
Pribylova, Jaroslava ;
Spak, Josef .
ARCHIVES OF VIROLOGY, 2018, 163 (12) :3439-3442
[10]   Interactive Tree of Life (iTOL) v6: recent updates to the phylogenetic tree display and annotation tool [J].
Letunic, Ivica ;
Bork, Peer .
NUCLEIC ACIDS RESEARCH, 2024, 52 (W1) :W78-W82