Flow cytometry for extracellular vesicle characterization in COVID-19 and post-acute sequelae of SARS-CoV-2 infection

被引：0
作者：
Fanelli, Marialaura [1 ]
Petrone, Vita [1 ]
Chirico, Rossella [1 ]
Radu, Claudia Maria [2 ]
Minutolo, Antonella [1 ]
Matteucci, Claudia [1 ]
机构：
[1] Univ Roma Tor Vergata, Dept Expt Med, Via Montpellier 1, I-00133 Rome, Italy
[2] Univ Padua, Dept Med DIMED, Thrombot & Hemorrhag Dis Unit, I-35128 Padua, Italy
来源：
EXTRACELLULAR VESICLES AND CIRCULATING NUCLEIC ACIDS | 2024年 / 5卷 / 03期
关键词：
Multidistrict infection; multiparametric analysis; biomarkers; flow cytometry; COVID-19; SARS-CoV-2; PASC; Long COVID; MECHANISMS;
D O I：
10.20517/evcna.2024.20
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Infection with SARS-CoV-2, the virus responsible for COVID-19 diseases, can impact different tissues and induce significant cellular alterations. The production of extracellular vesicles (EVs), which are physiologically involved in cell communication, is also altered during COVID-19, along with the dysfunction of cytoplasmic organelles. Since circulating EVs reflect the state of their cells of origin, they represent valuable tools for monitoring pathological conditions. Despite challenges in detecting EVs due to their size and specific cellular compartment origin using different methodologies, flow cytometry has proven to be an effective method for assessing the role of EVs in COVID-19. This review summarizes the involvement of plasmatic EVs in COVID-19 patients and individuals with Long COVID (LC) affected by post-acute sequelae of SARS-CoV-2 infection (PASC), highlighting their dual role in exerting both pro- and antiviral effects. We also emphasize how flow cytometry, with its multiparametric approach, can be employed to characterize circulating EVs, particularly in infectious diseases such as COVID-19, and suggest their potential role in chronic impairments during post-infection.
引用
收藏
页码：517 / 537
页数：21
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