Gata6 functions in zebrafish endoderm to regulate late differentiating arterial pole cardiogenesis

被引:1
作者
Sam, Jessica [1 ,2 ]
Torregroza, Ingrid [1 ]
Evans, Todd [1 ,3 ,4 ]
机构
[1] Weill Cornell Med, Dept Surg, New York, NY 10065 USA
[2] Weill Cornell Med, Grad Sch Med Sci, New York, NY 10065 USA
[3] Weill Cornell Med, Hartman Inst Therapeut Organ Regenerat, New York, NY 10065 USA
[4] Ctr Genom Hlth, Weill Cornell Med, New York, NY 10065 USA
来源
DEVELOPMENT | 2024年 / 151卷 / 16期
基金
美国国家卫生研究院;
关键词
Heart development; Morphogenesis; Congenital heart disease; FGF signaling; 2ND HEART FIELD; OUTFLOW TRACT; TRANSCRIPTION FACTORS; SPECIFICATION; MORPHOGENESIS; MESODERM; DEFECTS; GROWTH; PROLIFERATION; PROGENITORS;
D O I
10.1242/dev.202895
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Mutations in GATA6 are associated with congenital heart disease, most notably conotruncal structural defects. However, how GATA6 regulates cardiac morphology during embryogenesis is undefined. We used knockout and conditional mutant zebrafish alleles to investigate the spatiotemporal role of gata6 during cardiogenesis. Loss of gata6 specifically impacts atrioventricular valve formation and recruitment of epicardium, with a prominent loss of arterial pole cardiac cells, including those of the ventricle and outflow tract. However, there are no obvious defects in cardiac progenitor cell specification, proliferation or death. Conditional loss of gata6 starting at 24 h is sufficient to disrupt the addition of late differentiating cardiomyocytes at the arterial pole, with decreased expression levels of anterior secondary heart field (SHF) markers spry4 and mef2cb. Conditional loss of gata6 in the endoderm is sufficient to phenocopy the straight knockout, resulting in a significant loss of ventricular and outflow tract tissue. Exposure to a Dusp6 inhibitor largely rescues the loss of ventricular cells in gata6-/- - /- larvae. Thus, gata6 functions in endoderm are mediated by FGF signaling to regulate the addition of anterior SHF progenitor derivatives during heart formation.
引用
收藏
页数:13
相关论文
共 70 条
  • [1] In vivo analysis of quiescent adult neural stem cells responding to Sonic hedgehog
    Ahn, S
    Joyner, AL
    [J]. NATURE, 2005, 437 (7060) : 894 - 897
  • [2] Alsan BH, 2002, DEVELOPMENT, V129, P1935
  • [3] Inactivation of Tbx1 in the pharyngeal endoderm results in 22q11DS malformations
    Arnold, JS
    Werling, U
    Braunstein, EM
    Liao, J
    Nowotschin, S
    Edelmann, W
    Hebert, JM
    Morrow, BE
    [J]. DEVELOPMENT, 2006, 133 (05): : 977 - 987
  • [4] Aberrant differentiation of second heart field mesoderm prefigures cellular defects in the outflow tract in response to loss of FGF8
    Astrof, Sophie
    Arriagada, Cecilia
    Saijoh, Yukio
    Francou, Alexandre
    Kelly, Robert G.
    Moon, Anne
    [J]. DEVELOPMENTAL BIOLOGY, 2023, 499 : 10 - 21
  • [6] Evaluating High-Confidence Genes in Conotruncal Cardiac Defects by Gene Burden Analyses
    Chui, Martin M. C.
    Mak, Christopher C. Y.
    Yu, Mullin H. C.
    Wong, Sandra Y. Y.
    Lun, Kin-Shing
    Yung, Tak-Cheung
    Kwong, Anna K. Y.
    Chow, Pak-Cheong
    Chung, Brian H. Y.
    [J]. JOURNAL OF THE AMERICAN HEART ASSOCIATION, 2023, 12 (04):
  • [7] Distinct phases of cardiomyocyte differentiation regulate growth of the zebrafish heart
    de Pater, Emma
    Clijsters, Linda
    Marques, Sara R.
    Lin, Yi-Fan
    Garavito-Aguilar, Zayra V.
    Yelon, Deborah
    Bakkers, Jeroen
    [J]. DEVELOPMENT, 2009, 136 (10): : 1633 - 1641
  • [8] Gata6 is an important regulator of mouse pancreas development
    Decker, Kimberly
    Goldman, Devorah C.
    Grasch, Catherine L.
    Sussel, Lori
    [J]. DEVELOPMENTAL BIOLOGY, 2006, 298 (02) : 415 - 429
  • [9] Mef2c is a direct transcriptional target of ISL1 and GATA factors in the anterior heart field during mouse embryonic development
    Dodou, E
    Verzi, MP
    Anderson, JR
    Xu, SM
    Black, BL
    [J]. DEVELOPMENT, 2004, 131 (16): : 3931 - 3942
  • [10] In Vivo Performance and Properties of Tamoxifen Metabolites for CreERT2 Control
    Felker, Anastasia
    Nieuwenhuize, Susan
    Dolbois, Aymeric
    Blazkova, Kristyna
    Hess, Christopher
    Low, Larry W. L.
    Burger, Sibylle
    Samson, Natasha
    Carney, Tom J.
    Bartunek, Petr
    Nevado, Cristina
    Mosimann, Christian
    [J]. PLOS ONE, 2016, 11 (04):