Identification of genes used by Escherichia coli to mitigate climatic stress conditions

被引:0
作者
Roufou, Styliani [1 ]
Griffin, Sholeem [1 ,3 ]
Katsini, Lydia [2 ]
Polanska, Monika [2 ]
Van Impe, Jan F. M.
Alexiou, Panagiotis [4 ]
Valdramidis, Vasilis P. [1 ,3 ,5 ]
机构
[1] Univ Malta, Fac Hlth Sci, Dept Food Sci & Nutr, Msida MSD2080, Malta
[2] Katholieke Univ Leuven, Dept Chem Engn BioTeC Chem & Bioproc Technol & Con, B-9000 Ghent, Belgium
[3] Univ Malta, Ctr Mol Med & Biobanking, Msida MSD2080, Malta
[4] Univ Malta, Fac Hlth Sci, Dept Appl Biomed Sci, Msida MSD2080, Malta
[5] Univ Athens, Fac Sci, Dept Chem, Athens, Greece
来源
GENE REPORTS | 2024年 / 36卷
关键词
Escherichia coli; RNA-Seq; Climate change; Transcriptome analysis; Gene expression; TRANSPORT; PROVIDES;
D O I
10.1016/j.genrep.2024.101998
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Introduction: Increasing temperature and elevated atmospheric carbon dioxide levels could impact the survival of stress-adapted bacteria. Method and results: In this study, RNA sequencing was performed to quantify Escherichia coli BL21 (DE3) gene expression in culture media under environmental temperature (27 degrees C and 42 degrees C) and carbon dioxide (0.2 % and 6 % v/v) conditions. Differential gene expression analysis between low temperature and different carbon dioxide conditions revealed up-regulated genes such as ldtC and bioA, optimising cell wall integrity and nutrient uptake. While, the downregulation of genes such as malK suggested a metabolic adaptation. Low temperature and high carbon dioxide levels enhanced bacterial tolerance to environmental stress. Under optimal temperatures, Escherichia coli adapts through various metabolic pathways while engaging in different processes at low temperatures and carbon dioxide levels, with temperature having a greater impact on gene expression than carbon dioxide levels. This study shows that complex metabolic activities are activated when both temperatures and carbon dioxide levels are combinsed, affecting antimicrobial resistance, nutrient acquisition, and adaptation strategies. Conclusion: Each condition exhibited mechanisms to enhance cellular resilience and adaptation to stress, focusing on cellular integrity, optimising energy utilisation, and activating stress response pathways. Impact statement: The findings stress the importance of continued research to understand the implications of climate change on microbial transcriptomes comprehensively.
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页数:11
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