Effect of Acetylsalicylic Acid on Biological Properties of Novel Cement Based on Calcium Phosphate Doped with Ions of Strontium, Copper, and Zinc

被引：0

作者：

Tovilovic, Tamara Vlajic ^{[1
]}

Petrovic, Sanja ^{[1
]}

Lazarevic, Milos ^{[1
]}

Pavic, Aleksandar ^{[2
]}

Plackic, Nikola ^{[2
]}

Milovanovic, Aleksa ^{[3
]}

Milosevic, Milos ^{[3
]}

Miletic, Vesna ^{[4
]}

Veljovic, Djordje ^{[5
]}

Radunovic, Milena ^{[1
]}

机构：

[1] Univ Belgrade, Sch Dent Med, Belgrade 11000, Serbia

[2] Univ Belgrade, Inst Mol Genet & Genet Engn, Belgrade 11000, Serbia

[3] Univ Belgrade, Fac Mech Engn, Belgrade 11000, Serbia

[4] Univ Sydney, Fac Med & Hlth, Sydney Dent Sch, Surry Hills, NSW 2010, Australia

[5] Univ Belgrade, Fac Technol & Met, Belgrade 11000, Serbia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2024年 / 25卷 / 14期

关键词：

calcium phosphate; hydroxyapatite; antibiofilm; dental pulp stem cells; zebrafish; DEEP CARIES LESIONS; IN-VITRO EVALUATION; PULP STEM-CELLS; MECHANICAL-PROPERTIES; PHYSICAL-PROPERTIES; COMPLETE EXCAVATION; HYDROXIDE MATERIALS; ASPIRIN; SILICATE; BIOCOMPATIBILITY;

D O I：

10.3390/ijms25147940

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study aimed to compare the biological properties of newly synthesized cements based on calcium phosphate with a commercially used cement, mineral trioxide aggregate (MTA). Strontium (Sr)-, Copper (Cu)-, and Zinc (Zn)-doped hydroxyapatite (miHAp) powder was obtained through hydrothermal synthesis and characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectrometry (EDX). Calcium phosphate cement (CPC) was produced by mixing miHAp powder with a 20 wt.% citric acid solution, followed by the assessment of its compressive strength, setting time, and in vitro bioactivity. Acetylsalicylic acid (ASA) was added to the CPC, resulting in CPCA. Biological tests were conducted on CPC, CPCA, and MTA. The biocompatibility of the cement extracts was evaluated in vitro using human dental pulp stem cells (hDPSCs) and in vivo using a zebrafish model. Antibiofilm and antimicrobial effect (quantified by CFUs/mL) were assessed against Streptococcus mutans and Lactobacillus rhamnosus. None of the tested materials showed toxicity, while CPCA even increased hDPSCs proliferation. CPCA showed a better safety profile than MTA and CPC, and no toxic or immunomodulatory effects on the zebrafish model. CPCA exhibited similar antibiofilm effects against S. mutans and L. rhamnosus to MTA.

引用

页数：22