In Vivo Thoracic Dorsal Root Ganglia (DRG) Calcium Imaging and ECG Recording for Studying Peripheral Nerve Stimulation

被引:0
作者
Li, Xia [1 ]
Liu, Yun [1 ]
Liu, Kun [1 ]
Du, Longhua [1 ]
Lv, Tao [1 ]
Zhu, Bing [1 ]
Gao, Xinyan [1 ]
机构
[1] China Acad Chinese Med Sci, Inst Acupuncture & Moxibust, Beijing, Peoples R China
来源
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2024年 / 210期
基金
中国国家自然科学基金;
关键词
NEURONS; ACUPUNCTURE; EXPRESSION;
D O I
10.3791/67283
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The dorsal root ganglia (DRG), housing primary sensory neurons, transmit somatosensory and visceral afferent inputs to the dorsal horn of the spinal cord. They play a pivotal role in both physiological and pathological states, including neuropathic and visceral pain. In vivo calcium imaging of DRG enables real-time observation of calcium transients in single units or neuron ensembles. Accumulating evidence indicates that DRG neuronal activities induced by somatic stimulation significantly affect autonomic and visceral functions. While lumbar DRG calcium imaging has been extensively studied, thoracic segment DRG calcium imaging has been less explored due to surgical exposure and stereotaxic fixation challenges. Here, we utilized in vivo calcium imaging at the thoracic1 dorsal root ganglion (T1-DRG) to investigate changes in neuronal activity resulting from somatic stimulations of the forelimb. This approach is crucial for understanding the somato-cardiac reflex triggered by peripheral nerve stimulations (PENS), such as acupuncture. Notably, synchronization of cardiac function was observed and measured by electrocardiogram (ECG), with T-DRG neuronal activities, potentially establishing a novel paradigm for somato-visceral reflex in the thoracic segments.
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页数:9
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