Insect cell production of chimeric virus-like particles based on human immunodeficiency virus GAG proteins and yellow fever virus envelope protein

被引：0

作者：

Morgado, Fabricio da Silva ^{[1
]}

Cahu, Roberta ^{[1
]}

de Jesus, Daniela Carrilho ^{[1
]}

Chaves, Lorena Carvalho de Souza ^{[2
]}

Ribeiro, Bergmann Morais ^{[1
]}

机构：

[1] Univ Brasilia, Inst Biol, Dept Cell Biol, Brasilia, Brazil

[2] Emory Univ, Dept Biomed Engn, Atlanta, GA USA

来源：

BRAZILIAN JOURNAL OF MICROBIOLOGY | 2024年 / 55卷 / 04期

关键词：

Baculovirus; Virus-like particle; Yellow fever virus; Envelope; Human immunodeficiency virus; DOMAIN-III; 3-DIMENSIONAL STRUCTURE; N-GLYCOSYLATION; MATRIX PROTEIN; MEMBRANE; FUSION; PR55(GAG); GLYCOPROTEIN; EXPRESSION; BINDING;

D O I：

10.1007/s42770-024-01509-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The yellow fever virus (YFV) is a single stranded RNA virus belonging to the genus Orthoflavivirus that is capable of zoonotic transmissions that infect nonhuman and human primates. It is endemic in Brazil with recurrent epidemics of the disease, and it is transmitted through mosquitoes. The detection and immunization against YFV and other flaviviruses are fundamental for the management of the impacts of the disease in human environments. In an ongoing effort to develop new approaches for diagnostics and immunizations, we expressed VLPs displaying the yellow fever virus envelope protein (YFE) using recombinant baculovirus in insect cells. By co-expressing HIV-1 Pr55Gag protein (GAG) together with YFE we were able to generate chimeric VLPs containing a GAG core together with an envelope containing the YFE protein. The YFE and the chimeric GAG-YFE VLPs have potential as vaccine candidates and as reagents for serological assays in the detection of these viruses in human sera.

引用

页码：3187 / 3197

页数：11