Identification fi cation of hub genes and key pathways in arsenic-treated rice ( Oryza sativa L.) based on 9 topological analysis methods of CytoHubba

Background: Arsenic is a toxic metalloid that can cause acute and chronic adverse health problems. Unfortunately, rice, the primary staple food for more than half of the world's ' s population, is generally regarded as a typical arsenic-accumulating crop plant. Evidence indicates that arsenic stress can influence fl uence the growth and development of the rice plant, and lead to high concentrations of arsenic in rice grain. But the underlying mechanisms remain unclear. Methods: In the present research, the possible molecules and pathways involved in rice roots in response to arsenic stress were explored using bioinformatics methods. Datasets that involving arsenic-treated rice root and the " study type" " that was restricted to " Expression profiling fi ling by array" " were selected and downloaded from Gene Expression Omnibus (GEO) database. Differentially ff erentially expressed genes (DEGs) between the arsenic-treated group and the control group were obtained using the online web tool GEO2R. Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed to investigate the functions of DEGs. The protein-protein interactions (PPI) network and the molecular complex detection algorithm (MCODE) of DEGs were analyzed using STRING and Cystoscope, respectively. Important nodes and hub genes in the PPI network were predicted and explored using the Cytoscape-cytoHubba plug-in. Results: Two datasets, GSE25206 and GSE71492, were downloaded from Gene Expression Omnibus (GEO) database. Eighty common DEGs from the two datasets, including sixty-three up-regulated and seventeen down-regulated genes, were then selected. After functional enrichment analysis, these common DEGs were enriched mainly in 10 GO items, including glutathione transferase activity, glutathione metabolic process, toxin catabolic process, and 7 KEGG pathways related to metabolism. After PPI network and MCODE analysis, 49 nodes from the DEGs PPI network were identified, fi ed, fi ltering two significant fi cant modules. Next, the CytoscapecytoHubba plug-in was used to predict important nodes and hub genes. Finally, fi ve genes [Os0]g0644000, PRDX6 (Os07g0638400), PRX]]2 (Os07g0677300), ENO](Os06g0]36600), LOGL9 (Os09g0547500)] were verified fi ed and could serve as the best candidates associated with rice root in response to arsenic stress. Conclusions: In summary, we elucidated the potential pathways and genes in rice root in response to arsenic stress through a comprehensive bioinformatics analysis.