Mouse models of surgical and neuropathic pain produce distinct functional alterations to prodynorphin expressing neurons in the prelimbic cortex

被引:0
作者
Zhou, Shudi [1 ,2 ]
Yin, Yuexi [1 ,2 ]
Sheets, Patrick L. [1 ,2 ,3 ]
机构
[1] Indiana Univ Sch Med, Med Neurosci Grad Program, Indianapolis, IN 46202 USA
[2] Indiana Univ Sch Med, Stark Neurosci Res Inst, Indianapolis, IN 46202 USA
[3] Indiana Univ Sch Med, Dept Pharmacol & Toxicol, Indianapolis, IN 46202 USA
基金
美国国家卫生研究院;
关键词
Pain models; Prodynorphin; Electrophysiology; Brain slice; Prelimbic cortex; Sex differences; KAPPA-OPIOID RECEPTOR; MEDIAL PREFRONTAL CORTEX; PYRAMIDAL NEURONS; DORSAL RAPHE; DYNORPHIN; STRESS; RAT; CONNECTIVITY; ANTAGONISTS; ACTIVATION;
D O I
暂无
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
The medial prefrontal cortex (mPFC) consists of a heterogeneous population of neurons that respond to painful stimuli, and our understanding of how different pain models alter these specific mPFC cell types remains incomplete. A distinct subpopulation of mPFC neurons express prodynorphin (Pdyn + ), the endogenous peptide agonist for kappa opioid receptors (KORs). Here, we used whole cell patch clamp for studying excitability changes to Pdyn expressing neurons in the prelimbic region of the mPFC (PL Pdyn + neurons) in mouse models of surgical and neuropathic pain. Our recordings revealed that PL Pdyn + neurons consist of both pyramidal and inhibitory cell types. We find that the plantar incision model (PIM) of surgical pain increases intrinsic excitability only in pyramidal PL Pdyn + neurons one day after incision. Following recovery from incision, excitability of pyramidal PL Pdyn + neurons did not differ between male PIM and sham mice, but was decreased in PIM female mice. Moreover, the excitability of inhibitory PL Pdyn + neurons was increased in male PIM mice, but was with no difference between female sham and PIM mice. In the spared nerve injury model (SNI), pyramidal PL Pdyn + neurons were hyperexcitable at both 3 days and 14 days after SNI. However, inhibitory PL Pdyn + neurons were hypoexcitable at 3 days but hyperexcitable at 14 days after SNI. Our findings suggest different subtypes of PL Pdyn + neurons manifest distinct alterations in the development of different pain modalities and are regulated by surgical pain in a sex-specific manner. Our study provides information on a specific neuronal population that is affected by surgical and neuropathic pain.
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页数:16
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共 67 条
[1]   Release of endogenous dynorphin opioids in the prefrontal cortex disrupts cognition [J].
Abraham, Antony D. ;
Casello, Sanne M. ;
Schattauer, Selena S. ;
Wong, Brenden A. ;
Mizuno, Grace O. ;
Mahe, Karan ;
Tian, Lin ;
Land, Benjamin B. ;
Chavkin, Charles .
NEUROPSYCHOPHARMACOLOGY, 2021, 46 (13) :2330-2339
[2]   Trigeminal injury causes kappa opioid-dependent allodynic, glial and immune cell responses in mice [J].
Aita, Megumi ;
Byers, Margaret R. ;
Chavkin, Charles ;
Xu, Mei .
MOLECULAR PAIN, 2010, 6
[3]   An epigenetic hypothesis for the genomic memory of pain [J].
Alvarado, Sebastian ;
Tajerian, Maral ;
Suderman, Matthew ;
Machnes, Ziv ;
Pierfelice, Stephanie ;
Millecamps, Magali ;
Stone, Laura S. ;
Szyf, Moshe .
FRONTIERS IN CELLULAR NEUROSCIENCE, 2015, 9
[4]   Peripheral nerve injury is accompanied by chronic transcriptome-wide changes in the mouse prefrontal cortex [J].
Alvarado, Sebastian ;
Tajerian, Maral ;
Millecamps, Magali ;
Suderman, Mathew ;
Stone, Laura S. ;
Szyf, Moshe .
MOLECULAR PAIN, 2013, 9
[5]   EXPRESSION OF THE PRODYNORPHIN GENE IN THE DEVELOPING AND ADULT CEREBRAL-CORTEX OF THE RAT - AN INSITU HYBRIDIZATION STUDY [J].
ALVAREZBOLADO, G ;
FAIREN, A ;
DOUGLASS, J ;
NARANJO, JR .
JOURNAL OF COMPARATIVE NEUROLOGY, 1990, 300 (03) :287-300
[6]   Sublayer-specific microcircuits of corticospinal and corticostriatal neurons in motor cortex [J].
Anderson, Charles T. ;
Sheets, Patrick L. ;
Kiritani, Taro ;
Shepherd, Gordon M. G. .
NATURE NEUROSCIENCE, 2010, 13 (06) :739-U116
[7]  
BALSKUBIK R, 1993, J PHARMACOL EXP THER, V264, P489
[8]   Nerve Injury-Induced Neuropathic Pain Causes Disinhibition of the Anterior Cingulate Cortex [J].
Blom, Sigrid Marie ;
Pfister, Jean-Pascal ;
Santello, Mirko ;
Senn, Walter ;
Nevian, Thomas .
JOURNAL OF NEUROSCIENCE, 2014, 34 (17) :5754-5764
[9]   A simplified up-down method (SUDO) for measuring mechanical nociception in rodents using von Frey filaments [J].
Bonin, Robert P. ;
Bories, Cyril ;
De Koninck, Yves .
MOLECULAR PAIN, 2014, 10
[10]   Selective p38α MAPK Deletion in Serotonergic Neurons Produces Stress Resilience in Models of Depression and Addiction [J].
Bruchas, Michael R. ;
Schindler, Abigail G. ;
Shankar, Haripriya ;
Messinger, Daniel I. ;
Miyatake, Mayurni ;
Land, Benjamin B. ;
Lemos, Julia C. ;
Hagan, Catherine E. ;
Neumaier, John F. ;
Quintana, Albert ;
Palmiter, Richard D. ;
Chavkin, Charles .
NEURON, 2011, 71 (03) :498-511