Simultaneous determination of glyphosate, glufosinate and their metabolites in soybeans using solid-phase analytical derivatization and LC-MS/MS determination

被引：1

作者：

Sasano, Ryoichi ^{[1
,2
]}

Sekizawa, Junpei ^{[1
]}

Saito, Isao ^{[2
]}

Harano, Mikihisa ^{[1
]}

Katsumoto, Kyoka ^{[1
]}

Ito, Rie ^{[1
]}

Iwasaki, Yusuke ^{[1
]}

Taguchi, Takaaki ^{[3
]}

Tsutsumi, Tomoaki ^{[3
]}

Akiyama, Hiroshi ^{[1
,3
]}

机构：

[1] Hoshi Univ, Sch Pharm & Pharmaceut Sci, Dept Analyt Chem, 2 4 41 Ebara,Shinagawa Ku, Tokyo 1428501, Japan

[2] AiSTI SCI CO Ltd, 18 3 Arimoto, Wakayama, Wakayama 6408390, Japan

[3] Natl Inst Hlth Sci, 3 25 26 Tonomachi,Kawasaki Ku, Kawasaki, Kanagawa 2109501, Japan

来源：

FOOD CHEMISTRY-X | 2024年 / 24卷

关键词：

N-acetyl glyphosate; N-acetyl glufosinate; N-( tert-butyldimethylsilyl)- N-methyl trifluoroacetamide; Derivatization; Solid-phase analytical derivatization; LC-MS/MS; Glyphosate; N-(tert-butyldimethylsilyl)-N-methyl trifluoroacetamide; GAS-CHROMATOGRAPHY;

D O I：

10.1016/j.fochx.2024.101806

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Glyphosate and glufosinate are the most widely used herbicides worldwide. We developed a simple and rapid analytical method for detecting glyphosate, glufosinate, and their metabolites (N-acetyl glyphosate: Gly-A, Nacetyl glufosinate: Glu-A, and 3-(hydroxymethylphosphinyl)propanoic acid: MPPA) in soybeans. The method involved extraction with water, trapping in a mini-column containing polymer-based resin with strong anion exchange groups, dehydration with acetonitrile, and solid-phase analytical derivatization at ambient temperature for 1 min using N-(tert-butyldimethylsilyl)-N-methyl trifluoroacetamide (MTBSTFA), followed by Liquid chromatography-tandem mass spectrometry (LC-MS/MS) determination. This method offers a straightforward and rapid analysis, using on-solid phase dehydration and rapid derivatization at an ambient temperature with MTBSTFA, yielding reliable results for glyphosate, glufosinate, and their metabolites. The method was applied to both domestic and imported soybean samples. Glyphosate, glufosinate, and Glu-A were detected in imported feed soybeans and processed soybean meal for feed use, reflecting the current conditions of GM soybean cultivation.

引用

页数：7

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