Functional and Structural Analysis Reveals Distinct Biological Roles of Plant Synaptotagmins in Response to Environmental Stress

被引:0
|
作者
Garcia-Hernandez, Selene [1 ]
Rubio, Lourdes [2 ]
Rivera-Moreno, Maria [3 ]
Perez-Sancho, Jessica [1 ,4 ]
Morello-Lopez, Jorge [1 ]
del Valle, Alicia Esteban [1 ]
Benitez-Fuente, Francisco [1 ]
Beuzon, Carmen R. [5 ]
Macho, Alberto P. [4 ,5 ]
Ruiz-Lopez, Noemi [1 ]
Albert, Armando [3 ]
Botella, Miguel A. [1 ]
机构
[1] Univ Malaga, Area Mejora & Fisiol Plantas, Inst Hortofruticultura Subtrop & Mediterranea La M, Malaga, Spain
[2] Univ Malaga, Dept Bot & Fisiol Vegetal, Malaga, Spain
[3] Consejo Super Invest Cient IQF CSIC, Dept Cristalografia & Biol Estruct, Inst Quim Fis Blas Cabrera, Madrid, Spain
[4] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Ctr Plant Stress Biol, CAS Ctr Excellence Mol Plant Sci, Shanghai, Peoples R China
[5] Univ Malaga, Inst Hortofruticultura Subtrop & Mediterranea La M, Area Protecc Cultivo, Malaga, Spain
来源
PLANT CELL AND ENVIRONMENT | 2025年 / 48卷 / 01期
关键词
abiotic stress; Arabidopsis; biotic stress; C2; domain; contact sites; SMP domain; synaptotagmins; MEMBRANE CONTACT SITES; PLASMA-MEMBRANE; ARABIDOPSIS; ER; NETWORK; DOMAINS; TOMATO; ORIGIN;
D O I
10.1111/pce.15125
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Endoplasmic reticulum-plasma membrane contact sites (ER-PM CSs) are evolutionarily conserved membrane domains found in all eukaryotes, where the ER closely interfaces with the PM. This short distance is achieved in plants through the action of tether proteins such as synaptotagmins (SYTs). Arabidopsis comprises five SYT members (SYT1-SYT5), but whether they possess overlapping or distinct biological functions remains elusive. SYT1, the best-characterized member, plays an essential role in the resistance to abiotic stress. This study reveals that the functionally redundant SYT1 and SYT3 genes, but not SYT5, are involved in salt and cold stress resistance. We also show that, unlike SYT5, SYT1 and SYT3 are not required for Pseudomonas syringae resistance. Since SYT1 and SYT5 interact in vivo via their SMP domains, the distinct functions of these proteins cannot be caused by differences in their localization. Interestingly, structural phylogenetic analysis indicates that the SYT1 and SYT5 clades emerged early in the evolution of land plants. We also show that the SYT1 and SYT5 clades exhibit different structural features in their SMP and Ca(2+ )binding of their C2 domains, rationalizing their distinct biological roles.
引用
收藏
页码:260 / 271
页数:12
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