Suitable Promoter for DNA Vaccination Using a pDNA Ternary Complex

被引：1
作者：
Kurosaki, Tomoaki [1 ]
Nakamura, Hiroki [1 ]
Sasaki, Hitoshi [2 ]
Kodama, Yukinobu [3 ]
机构：
[1] Nagasaki Univ, Grad Sch Biomed Sci, 1-7-1 Sakamoto, Nagasaki 8528588, Japan
[2] Nagasaki Univ, Inst Trop Med, 1-12-4 Sakamoto, Nagasaki 8528523, Japan
[3] Nagasaki Univ Hosp, Dept Hosp Pharm, 1-7-1 Sakamoto, Nagasaki 8528501, Japan
来源：
PHARMACEUTICS | 2024年 / 16卷 / 05期
基金：
日本学术振兴会;
关键词：
promoters; gamma-polyglutamic acid; gene delivery; polyethylenimine; pDNA; POLY-L-LYSINE; GENE-EXPRESSION; PLASMID DNA; TRANSGENE EXPRESSION; IMMUNE-RESPONSES; DENDRITIC CELLS; DELIVERY; ANTIGEN; NANOPARTICLES; ENHANCER;
D O I：
10.3390/pharmaceutics16050679
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
In this study, we evaluated the effect of several promoters on the transfection activity and immune-induction efficiency of a plasmid DNA (pDNA)/polyethylenimine/gamma-polyglutamic acid complex (pDNA ternary complex). Model pDNAs encoding firefly luciferase (Luc) were constructed with several promoters, such as simian virus 40 (SV40), eukaryotic elongation factor 1 alpha (EF1), cytomegalovirus (CMV), and chicken beta actin hybrid (CBh) (pSV40-Luc, pEF1-Luc, pCMV-Luc, and pCBh-Luc, respectively). Four types of pDNA ternary complexes, each with approximately 145-nm particle size and -30-mV zeta-potential, were stably constructed. The pDNA ternary complex containing pSV40-Luc showed low gene expression, but the other complexes containing pEF1-Luc, pCMV-Luc, and pCBh-Luc showed high gene expression in DC2.4 cells and spleen after intravenous administration. After immunization using various pDNA encoding ovalbumin (OVA) such as pEF1-OVA, pCMV-OVA, and pCBh-OVA, only the pDNA ternary complex containing pCBh-OVA showed significant anti-OVA immunoglobulin G (IgG) induction. In conclusion, our results showed that the CBh promoter is potentially suitable for use in pDNA ternary complex-based DNA vaccination.
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页数：11
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