Complete genome sequence of the novel virulent phage PMBT24 infecting Enterocloster bolteae from the human gut

被引：1

作者：

Sprotte, Sabrina ^{[1
]}

Brinks, Erik ^{[1
]}

Neve, Horst ^{[1
]}

Franz, Charles M. A. P. ^{[1
]}

机构：

[1] Fed Res Inst Nutr & Food, Max Rubner Inst, Dept Microbiol & Biotechnol, Hermann Weigmann Str 1, D-24103 Kiel, Germany

来源：

HELIYON | 2024年 / 10卷 / 08期

关键词：

Novel phage PMBT24; Enterocloster bolteae; Complete genome; Human gut; CLOSTRIDIUM-BOLTEAE; EVOLUTION; NOV;

D O I：

10.1016/j.heliyon.2024.e28813

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

PMBT24, the first reported virulent bacteriophage infecting the anaerobic human gut bacterium Enterocloster bolteae strain MBT-21, was isolated from a municipal sewage sample and its genome was sequenced and analysed. Transmission electron microscopy revealed a phage with an icosahedral head and a long, non-contractile tail. The circularly permutated, 99,962-bp dsDNA genome of the pac-type phage has a mol% G + C content of 32.1 and comprises 173 putative ORFs. Using amino acid sequence-based phylogeny, phage PMBT24 showed similarity to other, hitherto non-published phage genomes in the databases. Our data suggested phage PMBT24 to present the type phage of a novel genus (proposed name Kielvirus) and novel family of phages (proposed name Kielviridae).

引用

页数：8

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