Protocol to detect senescence-associated β-galactosidase and immunoperoxidase activity in fresh-frozen murine tissues

被引：1

作者：

Idelfonso-Garcia, Osiris German ^{[1
,2
]}

Pacheco-Rivera, Ruth ^{[3
]}

Alarcon-Sanchez, Brisa Rodope ^{[1
,4
]}

Serrano-Luna, Jesas ^{[4
]}

Baltierrez-Hoyos, Rafael ^{[5
,6
]}

Vasquez-Garzon, Veronica Rocio ^{[5
,6
]}

Muriel, Pablo ^{[7
]}

Villa-Trevino, Saal ^{[4
]}

Perez-Carreon, Julio Isael ^{[1
]}

Arellanes-Robledo, Jaime ^{[1
,6
]}

机构：

[1] Natl Inst Genom Med INMEGEN, Lab Liver Dis, Mexico City 14610, Mexico

[2] Metropolitan Autonomous Univ Iztapalapa Campus UAM, Dept Hlth Sci, Mexico City 09340, Mexico

[3] Natl Polytech Inst, Natl Sch Biol Sci, Dept Biochem, Lab Mol Diagnost, Mexico City 07738, Mexico

[4] Natl Polytech Inst CINVESTAV IPN, Ctr Res & Adv Studies, Dept Cell Biol, Mexico City 07360, Mexico

[5] Benito Juarez Autonomous Univ Oaxaca, Fac Med & Surg, Lab Fibrosis & Canc, UABJO, Mexico City 68120, Mexico

[6] Natl Council Humanities Sci & Technol CONAHCYT, Humanist & Sci Res, Mexico City 03940, Mexico

[7] Natl Polytech Inst CINVESTAV IPN, Ctr Res & Adv Studies, Dept Pharmacol, Lab Expt Hepatol, Mexico City 07360, Mexico

来源：

STAR PROTOCOLS | 2024年 / 5卷 / 02期

关键词：

Antibody; Health Sciences; Microscopy;

D O I：

10.1016/j.xpro.2024.103009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Double labeling to identify different markers in the same tissue section represents a useful tool either for in situ diagnosis or characterization of molecular associations. Here, we present a protocol to detect senescence -associated 13-galac- tosidase (SA-13Gal) and immunoperoxidase (IPO) activity in fresh -frozen murine tissues. We describe steps for tissue collection, solution preparation, SA-13Gal staining, IPO staining, hematoxylin counterstaining, microscopic observation, and signal quantification. This protocol can be used to detect in situ proteins alongside SA-13Gal activity. For complete details on the use and execution of this protocol, please refer to Pacheco -Rivera et al.1

引用

页数：13

共 31 条

[31] Telomerase activity and telomerase subunits gene expression patterns in neuroblastoma: A molecular and immunohistochemical study establishing prognostic tools for fresh-frozen and paraffin-embedded tissues
Poremba, C
Scheel, C
Hero, B
Christiansen, H
Schaefer, KL
Nakayama, J
Berthold, F
Juergens, H
Boecker, W
Dockhorn-Dworniczak, B
JOURNAL OF CLINICAL ONCOLOGY, 2000, 18 (13) : 2582 - 2592

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