RNA Sequencing of Whole Blood in Premature Coronary Artery Disease: Identification of Novel Biomarkers and Involvement of T Cell Imbalance

被引：1

作者：

Chen, Si ^{[1
,2
,3
]}

Li, Zhan ^{[1
,2
]}

Li, Haolong ^{[1
,2
]}

Zeng, Xiaoli ^{[3
]}

Yuan, Hui ^{[3
]}

Li, Yongzhe ^{[1
,2
]}

机构：

[1] Chinese Acad Med Sci & Peking Union Med Coll, Dept Clin Lab, Peking Union Med Coll Hosp, 1 Shuaifuyuan, Beijing, Peoples R China

[2] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, State Key Lab Complex Severe & Rare Dis, Beijing, Peoples R China

[3] Capital Med Univ, Beijing Anzhen Hosp, Dept Clin Lab, Beijing, Peoples R China

来源：

JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH | 2024年 / 17卷 / 03期

关键词：

Premature coronary artery disease; RNA sequencing; T cells; MUC5B;

D O I：

10.1007/s12265-023-10465-8

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Serum biomarkers were explored based on the peripheral blood gene expression profiles of premature coronary artery disease (PCAD). RNA sequencing (RNA-Seq) was used to detect PCAD-specific differentially expressed genes (DEGs). Quantitative real-time polymerase chain reaction (RT-PCR) was used to validate the most significant DEGs, and enzyme-linked immunosorbent assay (ELISA) was utilized to quantify the effect on corresponding serum proteins. Fifty-nine PCAD-specific DEGs were identified. Functional analysis showed positive regulation of T cell-mediated cytotoxicity, regulation of T cell-mediated immunity, and the regulation of alpha-beta T cell proliferation which were enriched in PCAD. RT-PCR validated the significant difference in the expression of BAG6, MUC5B, and APOA2 between PCAD and late-onset coronary artery disease (LCAD) patients. ELISA validation showed serum MUC5B increased dramatically in PCAD when compared to LCAD. Our study found T cells contribute to the occurrence of PCAD, and the inflammatory factor MUC5B may be a novel serum marker in PCAD patients.

引用

页码：638 / 647

页数：10