The Role of Gold - Silver Nanocomposite Gel versus Astragalus Polysaccharides on Healing Process of Experimentally Induced Wound in Albino Rats Pharmacological and Histological Comparative Study

T HIS work aims to fabricate two alternative nanoparticle formulas based on natural materials ( Astragalus polysaccharides ) as well as metallic materials (gold and silver nanoparticles), which represent quick, simple, and cost-effective approaches for improving the activity of the particles to promote wound healing. The Gold -silver and APS Nanocomposite gels were characterized by using Transmission Electron Microscope (TEM), Zeta potential, dynamic light scattering. The results revealed spherical to sub -spherical shape of the Gold -Silver nanocomposite gel without any aggregation and with homogeneous dispersion in the gel matrix was shown in TEM images. , dynamic light scattering demonstrated that the size were 85 nm and (30-50 nm) for Gold -Silver nanocomposite gel and Astragalus polysaccharides nano gel respectively. The excellent bioactivity of the Gold -Silver nanocomposite gel and the Astragalus polysaccharides nano gel was demonstrated by their respective zeta potential values of -25.2 +/- 0.71 mV and +30.4 +/- 1.65 mV. The generated nanocomposite gel's capacity for in vivo wound repair was assessed by histopathological confirmation and macroscopical images that showed the healing process. In comparison to Gold -Silver nanocomposite gel and the untreated control group Pharmacological investigations wound size histopathological investigations of the Astragalus polysaccharides treated group revealed significantly greater re-epithelialization, hair follicle regeneration and nearly normal alpha-SMA. The generated Astragalus polysaccharides nano gel has better wound healing capability when compared to Gold -Silver nanocomposite gel and the untreated groups, according to our results of the investigated pharmacological parameters by increasing the wound closure at the 15 th day compared to other groups.