N ITROGEN (N) and phosphorus (P) released from different fertilizers to soil solution is a factor that affects soil fertility and plant growth. To evaluate the impacts of amending soil with organic, chemical and bio-fertilizers solely or in combinations for uprising N, P available contents in soil and increasing the growth of black cumin ( Nigella sativa L) plants. The consequences of these additives on soil chemical properties were a matter of concern herein. To attain this aim, incubation and a pot experiment was conducting at 100% urea formaldehyde, 50% level of adding rabbit manure + 50% urea formaldehyde with or without bio-fertilizer, control without fertilization and bio-fertilizer single in a randomized block design which considering six equations (Zero -order, first -order equation, second -order equation, Pseudo -second -order, power function, and parabolic diffusion model) were used to describe variations among released N and P with incubation time. The results demonstrated that using chemical, organic, and bio-fertilizer resulted in considerable decrease in soil pH and increases in organic matter (SOM) and nutrients availability (N and P) by time progress for all treatments. Compared to other equations, the pseudo -second models provided a better description of the kinetics of changes in released N and P contents with time, with R 2 ranging from 0.99 to 1.00. The results show that the application of 50% Rabbit manure + 50% Urea formaldehyde + Bio-fertilizer (RUBF) significantly enhanced parameters of black cumin plants such as shoot dry weight, plant height, and stem diameter, chlorophyll a, b, and carotenoids ratio, dry weight capsules, number of capsules, and seed weight/plant. Our study is useful when using rabbit manure, ureaform and biofertilizers. That can be improving soil chemical properties, supplying available nutrients, the release kinetics and the factors related to the release of nutrients from these fertilizers are essential in planning strategies of nutrient management, additionally, boosted the growth, plants quality, yield and photosynthetic pigments.