Identifying the suite of genes central to swimming in the biocontrol bacterium Pseudomonas protegens Pf-5

被引:1
作者
Fabian, B. K. [1 ,2 ]
Foster, C. [1 ,4 ]
Asher, A. [1 ]
Hassan, K. A. [1 ,2 ,3 ]
Paulsen, I. T. [1 ,2 ]
Tetu, S. G. [1 ,2 ]
机构
[1] Macquarie Univ, Sch Nat Sci, Sydney, Australia
[2] Macquarie Univ, ARC Ctr Excellence Synthet Biol, Sydney, Australia
[3] Univ Newcastle, Sch Environm & Life Sci, Newcastle, NSW, Australia
[4] Univ NSW, Ramaciotti Ctr Genom, Sydney, Australia
来源
MICROBIAL GENOMICS | 2024年 / 10卷 / 03期
基金
澳大利亚研究理事会;
关键词
biocontrol; motility; plant growth- promoting rhizobacteria; swimming; TraDIS; transposon insertion sequencing; SWARMING MOTILITY; BIOFILM FORMATION; FLUORESCENS PF-5; AERUGINOSA PAO1; FUNCTIONAL-CHARACTERIZATION; TRANSPOSON MUTAGENESIS; RESPONSE REGULATOR; COTTON SEEDLINGS; CELL-SHAPE; SYSTEM;
D O I
10.1099/mgen.0.001212
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Swimming motility is a key bacterial trait, important to success in many niches. Biocontrol bacteria, such as Pseudomonas protegens Pf - 5, are increasingly used in agriculture to control crop diseases, where motility is important for colonization of the plant rhizosphere. Swimming motility typically involves a suite of flagella and chemotaxis genes, but the specific gene set employed for both regulation and biogenesis can differ substantially between organisms. Here we used transposon- directed insertion site sequencing (TraDIS), a genome- wide approach, to identify 249 genes involved in P. protegens Pf - 5 swimming motility. In addition to the expected flagella and chemotaxis, we also identified a suite of additional genes important for swimming, including genes related to peptidoglycan turnover, O- antigen biosynthesis, cell division, signal transduction, c- di- GMP turnover and phosphate transport, and 27 conserved hypothetical proteins. Gene knockout mutants and TraDIS data suggest that defects in the Pst phosphate transport system lead to enhanced swimming motility. Overall, this study expands our knowledge of pseudomonad motility and highlights the utility of a TraDIS- based approach for analysing the functions of thousands of genes. This work sets a foundation for understanding how swimming motility may be related to the inconsistency in biocontrol bacteria performance in the field.
引用
收藏
页数:14
相关论文
共 98 条
  • [11] The Effect of pstS and phoB on Quorum Sensing and Swarming Motility in Pseudomonas aeruginosa
    Blus-Kadosh, Inna
    Zilka, Anat
    Yerushalmi, Gal
    Banin, Ehud
    [J]. PLOS ONE, 2013, 8 (09):
  • [12] Booth SC, 2019, bioRxiv
  • [13] Pseudomonas Flagella: Generalities and Specificities
    Bouteiller, Mathilde
    Dupont, Charly
    Bourigault, Yvann
    Latour, Xavier
    Barbey, Corinne
    Konto-Ghiorghi, Yoan
    Merieau, Annabelle
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (07)
  • [14] The flagellum in bacterial pathogens: For motility and a whole lot more
    Chaban, Bonnie
    Hughes, H. Velocity
    Beeby, Morgan
    [J]. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2015, 46 : 91 - 103
  • [15] Defects in D-Rhamnosyl Residue Biosynthetic Genes Affect Lipopolysaccharide Structure, Motility, and Cell-Surface Hydrophobicity in Pseudomonas syringae Pathovar glycinea Race 4
    Chiku, Kazuhiro
    Tsunemi, Kazuhiko
    Yamamoto, Masanobu
    Ohnishi-Kameyama, Mayumi
    Yoshida, Mitsuru
    Ishii, Tadashi
    Taguchi, Fumiko
    Iwaki, Masako
    Ichinose, Yuki
    Ono, Hiroshi
    [J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2013, 77 (03) : 505 - 510
  • [16] Choi U, 2022, Microbiology, DOI [10.1101/2022.09.06.506770, DOI 10.1101/2022.09.06.506770]
  • [17] Helical and rod-shaped bacteria swim in helical trajectories with little additional propulsion from helical shape
    Constantino, Maira A.
    Jabbarzadeh, Mehdi
    Fu, Henry C.
    Bansil, Rama
    [J]. SCIENCE ADVANCES, 2016, 2 (11):
  • [18] Transposon Insertion Sequencing Elucidates Novel Gene Involvement in Susceptibility and Resistance to Phages T4 and T7 in Escherichia coli O157
    Cowley, Lauren A.
    Low, Alison S.
    Pickard, Derek
    Boinett, Christine J.
    Dallman, Timothy J.
    Day, Martin
    Perry, Neil
    Gally, David L.
    Parkhill, Julian
    Jenkins, Claire
    Cain, Amy K.
    [J]. MBIO, 2018, 9 (04):
  • [19] A four-tiered transcriptional regulatory circuit controls flagellar biogenesis in Pseudomonas aeruginosa
    Dasgupta, N
    Wolfgang, MC
    Goodman, AL
    Arora, SK
    Jyot, J
    Lory, S
    Ramphal, R
    [J]. MOLECULAR MICROBIOLOGY, 2003, 50 (03) : 809 - 824
  • [20] The GacS sensor kinase controls phenotypic reversion of small colony variants isolated from biofilms of Pseudomonas aeruginosa PA14
    Davies, James A.
    Harrison, Joe J.
    Marques, Lyriam L. R.
    Foglia, Ginevra R.
    Stremick, Carol A.
    Storey, Douglas G.
    Turner, Raymond J.
    Olson, Merle E.
    Ceri, Howard
    [J]. FEMS MICROBIOLOGY ECOLOGY, 2007, 59 (01) : 32 - 46