Molecular characterization, antimicrobial resistance and invasion of epithelial cells by Streptococcus agalactiae strains isolated from colonized pregnant women and newborns in Rio de Janeiro, Brazil

被引：1

作者：

Pimentel, Bruna Alves da Silva ^{[1
]}

Lannes-Costa, Pamella Silva ^{[1
]}

Viana, Alice Slotfeldt ^{[2
]}

Santos, Gabriela da Silva ^{[1
]}

Leobons, Maria Beatriz Gerardin Poirot ^{[3
]}

Ferreira-Carvalho, Bernadete Teixeira ^{[2
]}

Nagao, Prescilla Emy ^{[1
]}

机构：

[1] Rio de Janeiro State Univ UERJ, Inst Biol Roberto Alcantara Gomes, Lab Mol Biol & Physiol Streptococci, BR-20550013 Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Dept Microbiol Med, BR-21941902 Rio De Janeiro, RJ, Brazil

[3] Secretaria Municipal Saude Rio de Janeiro, BR-20720292 Rio De Janeiro, Brazil

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 2024年 / 135卷 / 08期

关键词：

Streptococcus agalactiae; molecular characterization; epithelial cells; pregnant women; newborns; GROUP-B STREPTOCOCCUS; CAPSULAR TYPES; EPIDEMIOLOGY; VIRULENCE; PROTEINS;

D O I：

10.1093/jambio/lxae200

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims To evaluate the prevalence, molecular characteristics, antimicrobial susceptibility, and epithelial invasion of Streptococcus agalactiae strains isolated from pregnant women and newborns in Rio de Janeiro, Brazil.Methods and results A total of 67 S. agalactiae isolates, 48 isolates from pregnant women and 19 from neonates, were analyzed. Capsular type Ia and V were predominant (35.8%/each). The multilocus sequence typing analysis revealed the presence of 19 STs grouped into 6 clonal complexes with prevalence of CC17/40.3% and CC23/34.3%. The lmb and iag virulence genes were found in 100% of isolates. Four S. agalactiae strains, belonging to CC17/ST1249 and CC23/ST23, were able to adhere to A549 respiratory epithelial cells. Antimicrobial resistance was verified mainly to tetracycline (85%), erythromycin (70.8%), and clindamycin (58.3%). Four S. agalactiae isolates were multidrug resistant. The resistance genes tested were found in 92.5% of isolates for tetM, 58.2% for ermB, 28.4% for mefAE, and 10.4% for tetO.Conclusion The study showed a high prevalence of virulence and antimicrobial genes in S. agalactiae strains isolated from pregnant women and newborns, supporting the idea that continued surveillance is necessary to identify risk factors and perform long-term follow-up in pregnant women and neonates in Rio de Janeiro.

引用

页数：9