Next-generation probiotic candidates targeting intestinal health in weaned piglets: Both live and heat-killed Akkermansia muciniphila prevent pathological changes induced by enterotoxigenic Escherichia coli in the gut

被引：4

作者：

Lan, Cong ^{[1
]}

Li, Hua ^{[1
]}

Shen, Yuqing ^{[1
]}

Liu, Yang ^{[1
]}

Wu, Aimin ^{[1
]}

He, Jun ^{[1
]}

Cai, Jingyi ^{[1
]}

Tian, Gang ^{[1
]}

Mao, Xiangbing ^{[1
]}

Huang, Zhiqing ^{[1
]}

Yu, Bing ^{[1
]}

Zheng, Ping ^{[1
]}

Yu, Jie ^{[1
]}

Luo, Junqiu ^{[1
]}

Yan, Hui ^{[1
]}

Luo, Yuheng ^{[1
]}

机构：

[1] Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr, Minist Educ China, Key Lab Anim Dis Resistance Nutr & Feed,Minist Agr, Chengdu, Peoples R China

来源：

ANIMAL NUTRITION | 2024年 / 17卷

基金：

中国国家自然科学基金;

关键词：

Akkermansia muciniphila; Weaned piglet; Enterotoxigenic Escherichia coli challenge; Intestinal health; MICROBIOTA; IMMUNITY; CELLS; PIGS;

D O I：

10.1016/j.aninu.2024.01.007

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The use of next-generation probiotics (NGP) in pigs for combating diseases has been subject to limited research. Here we explored the potential of a well-known NGP candidate Akkermansia muciniphila targeting pig gut health. In the first screening experiment, we found that the abundance of A. muciniphila peaked at 14 d old but decreased at weaning (21 d old; P < 0.05), suggesting the weaning period may be an effective window for A. muciniphila intervention. Following that, 48 crossbred weaned pigs at 28 d old were randomly assigned to five groups: control (CON), high/low live A. muciniphila (HA/LA), and high/low heat-killed A. muciniphila (HIA/LIA). From 1 to 28 d old, the CON group received gastric infusion of anaerobic sterile saline every other day; the HA and LA groups were gavaged every other day with 1 x 10(10) CFU/5 mL and 5 x 10(8) CFU/5 mL live A. muciniphila, respectively; and the HIA and LIA groups were gavaged every other day with 1 x 10(10) CFU/5 mL and 5 x 10(8) CFU/5 mL heat-killed A. muciniphila, respectively. At d 29, pigs in the CON group were randomly and equally divided into two groups, one of which was named the enterotoxigenic Escherichia coli (ETEC) group, and all groups except CON received a 5-d ETEC challenge. The supplementation of A. muciniphila numerically reduced the diarrhea rate of weaned pigs compared to the pigs that only received the ETEC challenge (P = 0.57), but the LIA group had a higher diarrhea rate than the CON group (P < 0.05). Consistent with this, the supplementation of A. muciniphila improved the small intestinal morphology and structure, proportion of CD4(+) T lymphocytes in the blood, as well as the expression of genes related to intestinal barrier and antioxidant indices of pigs with ETEC challenge, especially for the LA group (P < 0.05). Meanwhile, A. muciniphila supplementation reduced the expression of ETEC virulence factor genes in the ileum and colon of pigs challenged by ETEC (P < 0.05). Therefore, A. muciniphila may protect the intestinal health of weaned piglets from damage caused by ETEC infection, but the effect may vary depending on the concentration and activity of A. muciniphila. (c) 2024 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.

引用

页码：110 / 122

页数：13