Non-Alcoholic Fatty Liver Disease Induced by Feeding Medium-Chain Fatty Acids Upregulates Cholesterol and Lipid Homeostatic Genes in Skeletal Muscle of Neonatal Pigs

被引:1
作者
Gerrard, Samuel D. [1 ]
Biase, Fernando H. [1 ]
Yonke, Joseph A. [1 ]
Yadav, Ravi [1 ]
Shafron, Anthony J. [1 ]
Sunny, Nishanth E. [2 ]
Gerrard, David E. [1 ]
El-Kadi, Samer W. [1 ]
机构
[1] Virginia Tech, Sch Anim Sci, Blacksburg, VA 24601 USA
[2] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA
关键词
gene expression; skeletal muscle; metabolism; NAFLD; neonatal pig; TRANSCRIPTION FACTORS; METABOLISM; CONSEQUENCES; CONSUMPTION; EXPRESSION; STEATOSIS; MASS;
D O I
10.3390/metabo14070384
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Non-alcoholic fatty liver disease (NAFLD) is a range of disorders characterized by lipid accumulation in hepatocytes. Although this spectrum of disorders is associated with adult obesity, recent evidence suggests that this condition could also occur independently of obesity, even in children. Previously, we reported that pigs fed a formula containing medium-chain fatty acids (MCFAs) developed hepatic steatosis and weighed less than those fed an isocaloric formula containing long-chain fatty acids (LCFAs). Our objective was to determine the association between NAFLD and the skeletal muscle transcriptome in response to energy and lipid intake. Neonatal pigs were fed one of three formulas: a control formula (CONT, n = 6) or one of two isocaloric high-energy formulas containing either long (LCFA, n = 6) or medium (MCFA, n = 6) chain fatty acids. Pigs were fed for 22 d, and tissues were collected. Body weight at 20 and 22 d was greater for LCFA-fed pigs than their CONT or MCFA counterparts (p < 0.005). Longissimus dorsi weight was greater for LCFA compared with MCFA, while CONT was intermediate (p < 0.05). Lean gain and protein deposition were greater for LCFA than for CONT and MCFA groups (p < 0.01). Transcriptomic analysis revealed 36 differentially expressed genes (DEGs) between MCFA and LCFA, 53 DEGs between MCFA and CONT, and 52 DEGs between LCFA and CONT (FDR < 0.2). Feeding formula high in MCFAs resulted in lower body and muscle weights. Transcriptomics data suggest that the reduction in growth was associated with a disruption in cholesterol metabolism in skeletal muscles.
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页数:14
相关论文
共 55 条
[1]   Skeletal Muscle Dysfunction in the Development and Progression of Nonalcoholic Fatty Liver Disease [J].
Altajar, Sarah ;
Baffy, Gyorgy .
JOURNAL OF CLINICAL AND TRANSLATIONAL HEPATOLOGY, 2020, 8 (04) :414-423
[2]   The Prevalence of Non-Alcoholic Fatty Liver Disease in Children and Adolescents: A Systematic Review and Meta-Analysis [J].
Anderson, Emma L. ;
Howe, Laura D. ;
Jones, Hayley E. ;
Higgins, Julian P. T. ;
Lawlor, Debbie A. ;
Fraser, Abigail .
PLOS ONE, 2015, 10 (10)
[3]   High-fat diet overfeeding promotes nondetrimental liver steatosis in female mice [J].
Arisqueta, Lino ;
Navarro-Imaz, Hiart ;
Labiano, Ibone ;
Rueda, Yuri ;
Fresnedo, Olatz .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2018, 315 (05) :G772-G780
[4]   CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].
BENJAMINI, Y ;
HOCHBERG, Y .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300
[5]   Nutrient regulation of gene expression by the sterol regulatory element binding proteins:: Increased recruitment of gene-specific coregulatory factors and selective hyperacetylation of histone H3 in vivo [J].
Bennett, MK ;
Osborne, TF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6340-6344
[6]  
Berg J.M., 2002, Biochemistry, V5th
[7]   Trimmomatic: a flexible trimmer for Illumina sequence data [J].
Bolger, Anthony M. ;
Lohse, Marc ;
Usadel, Bjoern .
BIOINFORMATICS, 2014, 30 (15) :2114-2120
[8]   Lipid Composition, Digestion, and Absorption Differences among Neonatal Feeding Strategies: Potential Implications for Intestinal Inflammation in Preterm Infants [J].
Burge, Kathryn ;
Vieira, Frederico ;
Eckert, Jeffrey ;
Chaaban, Hala .
NUTRIENTS, 2021, 13 (02) :1-19
[9]   Relations of Steatosis Type, Grade, and Zonality to Histological Features in Pediatric Nonalcoholic Fatty Liver Disease [J].
Carter-Kent, Christine ;
Brunt, Elizabeth M. ;
Yerian, Lisa M. ;
Alkhouri, Naim ;
Angulo, Paul ;
Kohli, Rohit ;
Ling, Simon C. ;
Xanthakos, Stavra A. ;
Whitington, Peter F. ;
Charatcharoenwitthaya, Phunchai ;
Yap, Jason ;
Lopez, Rocio ;
McCullough, Arthur J. ;
Feldstein, Ariel E. .
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, 2011, 52 (02) :190-197
[10]   Ensembl 2022 [J].
Cunningham, Fiona ;
Allen, James E. ;
Allen, Jamie ;
Alvarez-Jarreta, Jorge ;
Amode, M. Ridwan ;
Armean, Irina M. ;
Austine-Orimoloye, Olanrewaju ;
Azov, Andrey G. ;
Barnes, If ;
Bennett, Ruth ;
Berry, Andrew ;
Bhai, Jyothish ;
Bignell, Alexandra ;
Billis, Konstantinos ;
Boddu, Sanjay ;
Brooks, Lucy ;
Charkhchi, Mehrnaz ;
Cummins, Carla ;
Fioretto, Luca Da Rin ;
Davidson, Claire ;
Dodiya, Kamalkumar ;
Donaldson, Sarah ;
El Houdaigui, Bilal ;
El Naboulsi, Tamara ;
Fatima, Reham ;
Giron, Carlos Garcia ;
Genez, Thiago ;
Martinez, Jose Gonzalez ;
Guijarro-Clarke, Cristina ;
Gymer, Arthur ;
Hardy, Matthew ;
Hollis, Zoe ;
Hourlier, Thibaut ;
Hunt, Toby ;
Juettemann, Thomas ;
Kaikala, Vinay ;
Kay, Mike ;
Lavidas, Ilias ;
Le, Tuan ;
Lemos, Diana ;
Marugan, Jose Carlos ;
Mohanan, Shamika ;
Mushtaq, Aleena ;
Naven, Marc ;
Ogeh, Denye N. ;
Parker, Anne ;
Parton, Andrew ;
Perry, Malcolm ;
Pilizota, Ivana ;
Prosovetskaia, Irina .
NUCLEIC ACIDS RESEARCH, 2022, 50 (D1) :D988-D995