Fabrication and Testing of Nano Bio-silica, Hemp, and Bamboo Fibre-Reinforced Chitosan Bio-composite Material

This article investigates the mechanical, thermal, and biocompatible properties of chitosan-reinforced bamboo, hemp, and nano-bio-silica-reinforced composites (CP, CF, C1, C2, C3) using hand layup. Nano bio-silica at 1%, 2%, and 3% was used to fabricate C1, C2, and C3. C2 exhibited superior mechanical strengths, with a tensile strength of 142.44 MPa, flexural strength of 186.58 MPa, and compressive strength of 64.20 MPa. It demonstrated low thermal conductivity (0.2460 W/m-K) and minimal mass loss in TGA. MTT assays confirmed non-cytotoxicity for CP, CF, C1, C2, and C3, highlighting C2's potential as a biocompatible material against S. aureus gram -positive bacteria.