Vibrational circular dichroism of adenosine crystals

被引:3
作者
Krupova, Monika [2 ]
Leszczenko, Patrycja [1 ,3 ,4 ]
Sierka, Ewa [1 ,3 ,5 ]
Hamplova, Sara Emma [6 ]
Klepetarova, Blanka
Pelc, Radek [5 ]
Andrushchenko, Valery [1 ]
机构
[1] Czech Acad Sci, Inst Organ Chem & Biochem, Flemingovo namesti 2, Prague 16610, Czech Republic
[2] UiT Arctic Univ Norway, Hylleraas Ctr Quantum Mol Sci, Dept Chem, N-9037 Tromso, Norway
[3] Jagiellonian Univ, Fac Chem, Gronostajowa 2, PL-30387 Krakow, Poland
[4] Jagiellonian Univ, Doctoral Sch Exact & Nat Sci, Lojasiewicza 11, PL-30348 Krakow, Poland
[5] Charles Univ Prague, Fac Med 3, Ruska 87, Prague 10000, Czech Republic
[6] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, England
关键词
Chiroptical spectroscopy; Vibrational circular dichroism (VCD); Solid-state VCD (SD-VCD); Vibrational spectroscopy; Enhanced VCD; Supramolecular chirality; Supramolecular VCD; VCD artifacts; Crystal; Nucleosides; Adenosine; INFRARED-SPECTROSCOPY; DNA INTERACTION; NUCLEIC-ACIDS; SOLID-PHASE; IR-SPECTRA; ADENINE; VCD; OPTIMIZATION; MOLECULES; GEOMETRY;
D O I
10.1016/j.saa.2024.124381
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
Adenosine is one of the building blocks of nucleic acids and other biologically important molecules. Spectroscopic methods have been among the most utilized techniques to study adenosine and its derivatives. However, most of them deal with adenosine in solution. Here, we present the first vibrational circular dichroism (VCD) spectroscopic study of adenosine crystals in solid state. Highly regular arrangement of adenosine molecules in a crystal resulted in a strongly enhanced supramolecular VCD signal originating from long-range coupling of vibrations. The data suggested that adenosine crystals, in contrast to guanosine ones, do not imbibe atmospheric water. Relatively large dimensions of the adenosine crystals resulted in scattering and substantial orientational artifacts affecting the spectra. Several strategies for tackling the artifacts have been proposed and tested. Atypical features in IR absorption spectra of crystalline adenosine (e.g., extremely low absorption in mid-IR spectral range) were observed and attributed to refractive properties of adenosine crystals.
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页数:17
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