Multiple scattering suppression for in vivo optical coherence tomography measurement using the B-scan-wise multi-focus averaging method

被引:1
作者
Zhu, Yiqiang [1 ]
Zhu, Lida [1 ]
Lim, Yiheng [1 ]
Makita, Shuichi [1 ]
Guo, Yu [1 ]
Yasuno, Yoshiaki [1 ]
机构
[1] Univ Tsukuba, Computat Opt Grp, Tsukuba, Ibaraki, Japan
来源
BIOMEDICAL OPTICS EXPRESS | 2024年 / 15卷 / 07期
基金
日本学术振兴会;
关键词
RETINAL BLOOD-FLOW; ADAPTIVE OPTICS; MONTE-CARLO; CONTRAST; SIGNAL; MICROSCOPY; OCT;
D O I
10.1364/BOE.524894
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We demonstrate a method that reduces the noise caused by multi-scattering (MS) photons in an in vivo optical coherence tomography image. This method combines a specially designed image acquisition (i.e., optical coherence tomography scan) scheme and subsequent complex signal processing. For the acquisition, multiple cross-sectional images (frames) are sequentially acquired while the depth position of the focus is altered for each frame by an electrically tunable lens. In the signal processing, the frames are numerically defocus-corrected, and complex averaged. Because of the inconsistency in the MS-photon trajectories among the different electrically tunable lens-induced defocus, this averaging reduces the MS signal. Unlike the previously demonstrated volume-wise multi-focus averaging method, our approach requires the sample to remain stable for only a brief period, approximately 70 ms, thus making it compatible with in vivo imaging. This method was validated using a scattering phantom and in vivo unanesthetized small fish samples, and was found to reduce MS noise even for unanesthetized in vivo measurement.
引用
收藏
页码:4044 / 4064
页数:21
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