Different expression patterns of DNA methyltransferases during horse testis development

被引：0

作者：

Yi, Minna ^{[1
]}

Asgenbaatar, Nairag ^{[1
,2
]}

Wang, Xisheng ^{[1
,3
]}

Ulaangerel, Tseweendolmaa ^{[1
]}

Shen, Yingchao ^{[1
]}

Wen, Xin ^{[1
]}

Du, Ming ^{[1
]}

Dong, Xiaoling ^{[2
,4
]}

Dugarjav, Manglai ^{[1
]}

Bou, Gerelchimeg ^{[1
]}

机构：

[1] Inner Mongolia Agr Univ, Inner Mongolia Key Lab Equine Sci Res & Technol In, Hohhot, Peoples R China

[2] Da Bei Nong Grp Ruminat Technol Ruminat Acad Haidi, Beijing, Peoples R China

[3] Jining Med Univ, Collaborat Innovat Ctr Birth Defect Res & Transfor, Jining, Peoples R China

[4] China Agr Univ, Beijing, Peoples R China

来源：

GENE | 2024年 / 920卷

基金：

中国国家自然科学基金;

关键词：

DNA methyltransferases; DNMT1; DNMT3; Spermatogenetic cell stages; horse testes; GERM-CELL DEVELOPMENT; DE-NOVO METHYLATION; EPIGENETIC MODIFICATIONS; REPETITIVE SEQUENCES; SERTOLI-CELLS; STEM-CELLS; SPERM DNA; DNMT3A; SPERMATOGENESIS; MAINTENANCE;

D O I：

10.1016/j.gene.2024.148531

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

DNA methyltransferases (DNMTs) are important epigenetic modification during spermatogenesis. To further evaluate the pattern of DNMTs in horse testes during development, we investigated the expression and localization of DNMT1, DNMT3a and DNMT3b at different time points. The qRT-PCR results showed that DNMT1 expression was maintained in testes tissue from 6-month-old (0.5y) to 2-year-old (2y) of age and decreased after 3-year-old (3y) ( P < 0.01). The expression levels of DNMT3a and DNMT3b peaked in testes tissue at 3y ( P < 0.01). At 4-year-old (4y), the expression of DNMT3a and DNMT3b was decreased and became similar to that at 0.5y. Immunofluorescence of DNMT1, DNMT3a and DNMT3b on testis samples confirmed the differential expression and localization of these three DNA methylation transferases during horse development. Further molecular biological studies are needed to understand the implications of the expression patterns of these DNMTs in horse testes.

引用

页数：8

共 56 条

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