Topical Clonidine Accelerates Cutaneous Wound Healing in Diabetic Rats by Regulating the Expression of Related Cytokine Signaling

被引:1
作者
Phan, Thi-Chau-Loan [1 ]
Chiang, Yi-Syuan [2 ]
Su, Ming-Jai [3 ]
Liang, Yao-Jen [1 ,2 ]
Juang, Shiow-Jen [4 ]
机构
[1] Fu Jen Catholic Univ, Grad Inst Appl Sci & Engn, New Taipei 242, Taiwan
[2] Fu Jen Catholic Univ, Dept Life Sci, New Taipei 242, Taiwan
[3] Natl Taiwan Univ, Grad Inst Pharmacol, Taipei 106, Taiwan
[4] Chi Mei Med Ctr, Dept Dermatol, Tainan 710, Taiwan
关键词
clonidine; wound healing; VEGF; SOCS3; JAK/STAT pathway; GROWTH-HORMONE; NITRIC-OXIDE; ACTIVATION; PAIN;
D O I
10.3390/cimb46060339
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Based on the analgesic and anti-inflammatory effects of clonidine in previous studies, we hypothesized that clonidine could accelerate wound healing in rats by regulating the expression of related cytokines. In this study, the wound healing effect of clonidine was evaluated using an excision wound model in diabetic rats and a HaCaT cell model. The wounds were treated daily with topical clonidine. The results analyzed by ImageJ2 software show that the wounds of the rats that were treated with 15 ng/mL clonidine recovered faster, and the wound size was also significantly reduced compared to the control group. Western blot assays determined that clonidine induced an increase in the expression of vascular growth factors, namely, Ang-1, Ang-2, and VEGF. Moreover, clonidine demonstrated a rescuing effect on JAK2 within the JAK/STAT pathway by inhibiting SOCS3 expression, leading to decreased SOCS3 levels and increased expression of JAK2 and phospho-STAT3. Histopathological analysis revealed that clonidine promoted complete epithelial repair and minimized inflammation in skin tissue. Additionally, clonidine stimulated HaCaT cell proliferation in vitro and enhanced cellular energy levels in the presence of AGEs. In conclusion, clonidine promoted vascular growth and wound healing by stimulating the expression of cytokines that are beneficial for wound healing.
引用
收藏
页码:5668 / 5681
页数:14
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