Serological Surveillance Development for Tropical Infectious Diseases Using Simultaneous Microsphere-Based Multiplex Assays and Finite Mixture Models

被引：33

作者：

Fujii, Yoshito ^{[1
]}

Kaneko, Satoshi ^{[1
,2
,3
]}

Nzou, Samson Muuo ^{[3
,4
]}

Mwau, Matilu ^{[4
]}

Njenga, Sammy M. ^{[5
]}

Tanigawa, Chihiro ^{[1
]}

Kimotho, James ^{[6
]}

Mwangi, Anne Wanjiru ^{[6
]}

Kiche, Ibrahim ^{[3
,7
]}

Matsumoto, Sohkichi ^{[8
]}

Niki, Mamiko ^{[8
]}

Osada-Oka, Mayuko ^{[8
,9
]}

Ichinose, Yoshio ^{[3
,10
]}

Inoue, Manabu ^{[8
]}

Itoh, Makoto ^{[11
]}

Tachibana, Hiroshi ^{[12
]}

Ishii, Kazunari ^{[13
]}

Tsuboi, Takafumi ^{[14
,15
]}

Yoshida, Lay Myint

Mondal, Dinesh ^{[16
]}

Haque, Rashidul ^{[16
]}

Hamano, Shinjiro ^{[3
,17
]}

Changoma, Mwatasa ^{[3
]}

Hoshi, Tomonori ^{[1
]}

Kamo, Ken-ichi ^{[18
]}

Karama, Mohamed ^{[2
,19
]}

Miura, Masashi ^{[1
]}

Hirayama, Kenji ^{[20
]}

机构：

[1] Nagasaki Univ NUITM, Inst Trop Med, Dept Ecoepidemiol, Nagasaki, Japan

[2] Nagasaki Univ, Grad Sch Int Hlth Dev, Nagasaki 852, Japan

[3] Nagasaki Univ, Inst Trop Med, Kenya Med Res Inst KEMRI, Nairobi, Kenya

[4] Kenya Med Res Inst KEMRI, Ctr Infect & Parasit Dis Control Res, Busia, Kenya

[5] Kenya Med Res Inst KEMRI, Eastern & Southern Africa Ctr Int Parasite Contro, Nairobi, Kenya

[6] Kenya Med Res Inst KEMRI, Prod Dept, Nairobi, Kenya

[7] ICIPE, Mbita, Mbita, Kenya

[8] Osaka City Univ, Grad Sch Med, Dept Bacteriol, Osaka 558, Japan

[9] Kyoto Prefectural Univ, Grad Sch Life & Environm Sci, Food Hyg & Environm Hlth Div Appl Life Sci, Kyoto 606, Japan

[10] Nagasaki Univ, Kenya Res Stn, Nagasaki 852, Japan

[11] Aichi Med Univ, Sch Med, Dept Infect & Immunol, Nagakute, Aichi 48011, Japan

[12] Tokai Univ, Sch Med, Dept Infect Dis, Hiratsuka, Kanagawa 25912, Japan

[13] Fukuoka Univ, Fac Med, Dept Microbiol & Immunol, Fukuoka 81401, Japan

[14] Ehime Univ, Div Malaria Res, Proteosci Ctr, Matsuyama, Ehime 790, Japan

[15] Nagasaki Univ, Inst Trop Med, Dept Paediat Infect Dis, Nagasaki 852, Japan

[16] ICDDR, Dhaka, Bangladesh

[17] Nagasaki Univ, Inst Trop Med, Dept Parasitol, Nagasaki 852, Japan

[18] Sapporo Med Univ, Dept Liberal Arts & Sci, Sapporo, Hokkaido, Japan

[19] Kenya Med Res Inst KEMRI, Ctr Publ Hlth Res, Nairobi, Kenya

[20] Nagasaki Univ, Inst Trop Med, Dept Immunogenet, Nagasaki 852, Japan

来源：

PLOS NEGLECTED TROPICAL DISEASES | 2014年 / 8卷 / 07期

关键词：

SUB-SAHARAN AFRICA; MYCOBACTERIUM-TUBERCULOSIS; VISCERAL LEISHMANIASIS; DEVELOPING-COUNTRIES; ESCHERICHIA-COLI; RISK-FACTORS; ANTIBODIES; SERODIAGNOSIS; PREVALENCE; DIAGNOSIS;

D O I：

10.1371/journal.pntd.0003040

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background: A strategy to combat infectious diseases, including neglected tropical diseases (NTDs), will depend on the development of reliable epidemiological surveillance methods. To establish a simple and practical seroprevalence detection system, we developed a microsphere-based multiplex immunoassay system and evaluated utility using samples obtained in Kenya. Methods: We developed a microsphere-based immuno-assay system to simultaneously measure the individual levels of plasma antibody (IgG) against 8 antigens derived from 6 pathogens: Entamoeba histolytica (C-IgL), Leishmania donovani (KRP42), Toxoplasma gondii (SAG1), Wuchereria bancrofti (SXP1), HIV (gag, gp120 and gp41), and Vibrio cholerae (cholera toxin). The assay system was validated using appropriate control samples. The assay system was applied for 3411 blood samples collected from the general population randomly selected from two health and demographic surveillance system (HDSS) cohorts in the coastal and western regions of Kenya. The immunoassay values distribution for each antigen was mathematically defined by a finite mixture model, and cut-off values were optimized. Findings: Sensitivities and specificities for each antigen ranged between 71 and 100%. Seroprevalences for each pathogen from the Kwale and Mbita HDSS sites (respectively) were as follows: HIV, 3.0% and 20.1%; L. donovani, 12.6% and 17.3%; E. histolytica, 12.8% and 16.6%; and T. gondii, 30.9% and 28.2%. Seroprevalences of W. bancrofti and V. cholerae showed relatively high figures, especially among children. The results might be affected by immunological cross reactions between W. bancrofti-SXP1 and other parasitic infections; and cholera toxin and the enterotoxigenic E. coli (ETEC), respectively. Interpretation: A microsphere-based multi-serological assay system can provide an opportunity to comprehensively grasp epidemiological features for NTDs. By adding pathogens and antigens of interest, optimized made-to-order high-quality programs can be established to utilize limited resources to effectively control NTDs in Africa.

引用

页数：15

共 46 条

[1]

[Anonymous], 2012, ANN SURV DAT NOT DIS

[2] Establishment of diagnostic cutoff points for levels of serum antibodies to pertussis toxin, filamentous hemagglutinin, and fimbriae in adolescents and adults in the United States [J].

Baughman, AL ;

Bisgard, KM ;

Edwards, KM ;

Guris, D ;

Decker, MD ;

Holland, K ;

Meade, BD ;

Lynn, F .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2004, 11 (06) :1045-1053

[3] IMMUNE ACTIVATION IS A DOMINANT FACTOR IN THE PATHOGENESIS OF AFRICAN AIDS [J].

BENTWICH, Z ;

KALINKOVICH, A ;

WEISMAN, Z .

IMMUNOLOGY TODAY, 1995, 16 (04) :187-191

[4] Simultaneous detection of antibodies to foot-and-mouth disease non-structural proteins 3ABC, 3D, 3A and 3B by a multiplexed Luminex assay to differentiate infected from vaccinated cattle [J].

Clavijo, A ;

Hole, K ;

Li, MY ;

Collignon, B .

VACCINE, 2006, 24 (10) :1693-1704

[5]

Deb P., 2007, FMM STATA MODULE EST

[6]

Department of Control of Neglected Tropical Diseases, 2006, NEGL TROP DIS

[7] EVALUATION OF A RECOMBINANT PARASITE ANTIGEN FOR THE DIAGNOSIS OF LYMPHATIC FILARIASIS [J].

DISSANAYAKE, S ;

ZHENG, HJ ;

DREYER, G ;

XU, M ;

WATAWANA, L ;

CHENG, GH ;

WANG, SH ;

MORIN, P ;

DENG, BJ ;

KURNIAWAN, L ;

VINCENT, A ;

PIESSENS, WF .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1994, 50 (06) :727-734

[8]

Everitt B S, 1996, Stat Methods Med Res, V5, P107, DOI 10.1177/096228029600500202

[9] DIAGNOSIS OF AIDS BY USING A 12-AMINO ACID PEPTIDE REPRESENTING AN IMMUNODOMINANT EPITOPE OF THE HUMAN-IMMUNODEFICIENCY-VIRUS [J].

GNANN, JW ;

SCHWIMMBECK, PL ;

NELSON, JA ;

TRUAX, AB ;

OLDSTONE, MBA .

JOURNAL OF INFECTIOUS DISEASES, 1987, 156 (02) :261-267

[10] Development of a fluorescent-microsphere immunoassay for detection of antibodies specific to equine arteritis virus and comparison with the virus neutralization test [J].

Go, Yun Young ;

Wong, Susan J. ;

Branscum, Adam J. ;

Demarest, Valerie L. ;

Shuck, Kathleen M. ;

Vickers, Mary L. ;

Zhang, Jianqiang ;

McCollum, William H. ;

Timoney, Peter J. ;

Balasuriya, Udeni B. R. .

CLINICAL AND VACCINE IMMUNOLOGY, 2008, 15 (01) :76-87

← 1 2 3 4 5 →