Developmental toxicity and mechanism of dibutyl phthalate and alternative diisobutyl phthalate in the early life stages of zebrafish (Danio rerio)

被引:3
作者
Tao, Huan-Yu [1 ,2 ]
Shi, Jianghong [1 ]
Zhang, Jiawei [1 ,2 ]
Ge, Hui [1 ]
Zhang, Mengtao [3 ]
Li, Xiao-Yan [2 ,3 ]
机构
[1] Southern Univ Sci & Technol, Sch Environm Sci & Engn, Shenzhen 518055, Peoples R China
[2] Univ Hong Kong, Environm Engn Res Ctr, Dept Civil Engn, Hong Kong, Peoples R China
[3] Tsinghua Univ, Grad Sch Shenzhen, Key Lab Microorganism Applicat & Risk Control Shen, Shenzhen 518055, Peoples R China
关键词
Dibutyl phthalate; Diisobutyl phthalate; Developmental toxicity; Joint toxicity; Thyroid hormone; Gene transcription; EXPRESSION; PESTICIDES; HORMONE; ESTERS; GENES; DBP; PCR;
D O I
10.1016/j.aquatox.2024.106962
中图分类号
Q17 [水生生物学];
学科分类号
071004 ;
摘要
Diisobutyl phthalate (DiBP), is widely chemical replacement for Dibutyl phthalate (DBP). Although DBP and DiBP have been detected in surface water worldwide, few studies to date have systematically assessed the risks of DBP and its alternatives to aquatic organisms. The present study compared DBP and DiBP for their individual and joint toxicity as well as thyroid hormone levels in zebrafish embryo. Transcripts of key genes related to the hypothalamic-pituitary-thyroid (HPT) axis were investigated in developing zebrafish larvae by application of real time polymerase chain reaction. The median half-lethal concentrations of DBP and DiBP to zebrafish at 96 h were 0.545 mg L-1 and 1.149 mg L-1, respectively. The joint toxic effect of DBP-DiBP (0.25-0.53 mg L-1) with the same ratio showed a synergistic effect. Thyroid hormones levels increased with exposure to 10 mu g L-1 of DBP or 50 mu g L-1 of DiBP, and exposure to both compounds significantly increased thyroid gland-specific transcription of thyroglobulin gene (tg), hyronine deiodinase (dio2), and transthyretin (ttr), indicating an adverse effect associated with the HPT axis. Molecular docking results indicated that DBP (-7.10 kcal/M and -7.53 kcal/M) and DiBP (-6.63 kcal/M and -7.42 kcal/M) had the same docking energy with thyroid hormone receptors. Our data facilities an understand of potential harmful effects of DBP and its alternative (DiBP).
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页数:9
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