Cd exposure confers (3-cypermethrin tolerance in Lymantria dispar by activating the ROS/CnCC signaling pathway-mediated P450 detoxification

被引：1

作者：

Tan, Mingtao ^{[1
,2
]}

Jiang, Hong ^{[1
,2
]}

Chai, Rusong ^{[3
]}

Fan, Miao ^{[3
]}

Niu, Zengting ^{[1
,2
]}

Sun, Guotong ^{[1
,2
]}

Yan, Shanchun ^{[1
,2
]}

Jiang, Dun ^{[1
,2
]}

机构：

[1] Northeast Forestry Univ, Sch Forestry, Harbin 150040, Peoples R China

[2] Northeast Forestry Univ, Key Lab Sustainable Forest Ecosyst Management, Minist Educ, Harbin 150040, Peoples R China

[3] Forest Bot Garden Heilongjiang Prov, Harbin 150040, Peoples R China

来源：

JOURNAL OF HAZARDOUS MATERIALS | 2024年 / 478卷

基金：

中国国家自然科学基金;

关键词：

Cadmium; Insecticide; Cross-tolerance; Lymantria dispar; Transcriptional regulatory mechanism; CADMIUM; RESISTANCE; GROWTH; ZN;

D O I：

10.1016/j.jhazmat.2024.135566

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Heavy metal pollutants are important abiotic environmental factors affecting pest habitats. In this study, Cd preexposure significantly increased the tolerance of Lymantria dispar larvae to (3-cypermethrin, but did not significantly alter their tolerance to 2-cyhalothrin and bifenthrin. The activation of P450 by Cd exposure is the key mechanism that induces insecticide cross-tolerance in L. dispar larvae. Both before and after (3-cypermethrin treatment, Cd exposure significantly increased the expression of CYP6AB224 and CYP6AB226 in L. dispar larvae. Silencing CYP6AB224 and CYP6AB226 reduced the tolerance of Cd-treated L. dispar larvae to (3-cypermethrin. Transgenic CYP6AB224 and CYP6AB226 genes significantly increased the tolerance of Drosophila and Sf9 cells to (3-cypermethrin, and the recombinant proteins of both genes could significantly metabolise (3-cypermethrin. Cd exposure significantly increased the expression of CnCC and Maf. CnCC was found to be a key transcription factor regulating CYP6AB224- and CYP6AB226-activated insecticide cross-tolerance in Cd-treated larvae. Decreasing reactive oxygen species (ROS) levels in the Cd-treated larvae or increasing ROS levels in the untreated larvae reduced or enhanced the expression of CnCC, CYP6AB224 and CYP6AB226 and (3-cypermethrin tolerance in L. dispar larvae, respectively. Collectively, Cd exposure confers beta- cypermethrin tolerance in L. dispar larvae through the ROS/CnCC CnCC signalling pathway-mediated P450 detoxification.

引用

页数：14