A molecular switch controls assembly of bacterial focal adhesions

被引：3

作者：

Attia, Bouchra ^{[1
]}

My, Laetitia ^{[2
]}

Castaing, Jean Philippe ^{[2
]}

Dinet, Celine ^{[2
]}

Le Guenno, Hugo ^{[3
]}

Schmidt, Victoria ^{[1
]}

Espinosa, Leon ^{[2
]}

Anantharaman, Vivek ^{[4
]}

Aravind, L. ^{[4
]}

Sebban-Kreuzer, Corinne ^{[1
]}

Nouailler, Matthieu ^{[1
]}

Bornet, Olivier ^{[3
]}

Viollier, Patrick ^{[5
]}

Elantak, Latifa ^{[1
]}

Mignot, Tam ^{[2
]}

机构：

[1] Aix Marseille Univ, CNRS, Inst Microbiol Mediterranee IMM, Lab Ingn Syst Macromol LISM,UMR 7255, 31 Chem Joseph Aiguier,CS 70071, F-13402 Marseille 20, France

[2] Aix Marseille Univ, Inst Microbiol Mediterranee IMM, Turing Ctr Living Syst, Lab Chim Bacterienne LCB,CNRS,UMR 7283, 31 Chem Joseph Aiguier CS70071, F-13402 Marseille 20, France

[3] Aix Marseille Univ, CNRS, Inst Microbiol Mediterranee IMM, UMR 7283, 31 Chem Joseph Aiguier,CS70071, F-13402 Marseille 20, France

[4] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA

[5] Univ Geneva, Fac Med, Ctr Med Univ, Dept Microbiol & Mol Med, CH-1211 Geneva 04, Switzerland

来源：

SCIENCE ADVANCES | 2024年 / 10卷 / 22期

关键词：

GLIDING MOTILITY; RAS HOMOLOG; G-PROTEIN; MREB; CYTOSKELETON; POLARITY; MODEL; GYF;

D O I：

10.1126/sciadv.adn2789

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cell motility universally relies on spatial regulation of focal adhesion complexes (FAs) connecting the substrate to cellular motors. In bacterial FAs, the Adventurous gliding motility machinery (Agl-Glt) assembles at the leading cell pole following a Mutual gliding-motility protein (MglA)-guanosine 5 '-triphosphate (GTP) gradient along the cell axis. Here, we show that GltJ, a machinery membrane protein, contains cytosolic motifs binding MglA-GTP and AglZ and recruiting the MreB cytoskeleton to initiate movement toward the lagging cell pole. In addition, MglA-GTP binding triggers a conformational shift in an adjacent GltJ zinc-finger domain, facilitating MglB recruitment near the lagging pole. This prompts GTP hydrolysis by MglA, leading to complex disassembly. The GltJ switch thus serves as a sensor for the MglA-GTP gradient, controlling FA activity spatially.

引用

页数：15

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