Genome resequencing reveals population divergence and local adaptation of blacklegged ticks in the United States

被引:4
作者
Schoville, Sean D. [1 ]
Burke, Russell L. [2 ]
Dong, Dahn-young [3 ]
Ginsberg, Howard S. [4 ]
Maestas, Lauren [5 ]
Paskewitz, Susan M. [1 ]
Tsao, Jean I. [6 ,7 ]
机构
[1] Univ Wisconsin Madison, Dept Entomol, 1630 Linden Dr, Madison, WI 53706 USA
[2] Hofstra Univ, Dept Biol, Hempstead, NY 11550 USA
[3] Univ Wisconsin Madison, Dept Integrat Biol, Madison, WI USA
[4] US Geol Survey, Univ Rhode Isl, Eastern Ecol Sci Ctr, Woodward Hall PSE,Field Stn, Kingston, RI 37919 USA
[5] USDA ARS, Cattle Fever Tick Res Lab, Edinburg, TX USA
[6] Michigan State Univ, Dept Fisheries & Wildlife, E Lansing, MI USA
[7] Michigan State Univ, Dept Large Anim Clin Sci, E Lansing, MI USA
关键词
deer ticks; Ixodes scapularis; Lyme disease; natural selection; population genomics; IXODES-SCAPULARIS ACARI; RECEPTOR-RELATED PROTEIN-1; SPONTANEOUS MUTATION-RATE; LYME-DISEASE; BORRELIA-BURGDORFERI; DROSOPHILA-MELANOGASTER; GENETIC-STRUCTURE; RANGE EXPANSION; DAMMINI ACARI; F-STATISTICS;
D O I
10.1111/mec.17460
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tick vectors and tick-borne disease are increasingly impacting human populations globally. An important challenge is to understand tick movement patterns, as this information can be used to improve management and predictive modelling of tick population dynamics. Evolutionary analysis of genetic divergence, gene flow and local adaptation provides insight on movement patterns at large spatiotemporal scales. We develop low coverage, whole genome resequencing data for 92 blacklegged ticks, Ixodes scapularis, representing range-wide variation across the United States. Through analysis of population genomic data, we find that tick populations are structured geographically, with gradual isolation by distance separating three population clusters in the northern United States, southeastern United States and a unique cluster represented by a sample from Tennessee. Populations in the northern United States underwent population contractions during the last glacial period and diverged from southern populations at least 50 thousand years ago. Genome scans of selection provide strong evidence of local adaptation at genes responding to host defences, blood-feeding and environmental variation. In addition, we explore the potential of low coverage genome sequencing of whole-tick samples for documenting the diversity of microbial pathogens and recover important tick-borne pathogens such as Borrelia burgdorferi. The combination of isolation by distance and local adaptation in blacklegged ticks demonstrates that gene flow, including recent expansion, is limited to geographical scales of a few hundred kilometres.
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页数:18
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