In vivo absorption, in vitro simulated digestion and fecal fermentation properties of polysaccharides from Pinelliae Rhizoma Praeparatum Cum Alumine and their effects on human gut microbiota

被引:6
|
作者
Gao, Kui-Xu [1 ]
Peng, Xi [1 ]
Wang, Jing-Ya [1 ]
Wang, Yao [1 ]
Pei, Ke [1 ]
Meng, Xiang-Long [1 ]
Zhang, Shuo-Sheng [1 ]
Hu, Mei-Bian [1 ]
Liu, Yu-Jie [1 ]
机构
[1] Shanxi Univ Chinese Med, Sch Tradit Chinese Med & Food Engn, Shanxi Prov Key Lab Tradit Chinese Med Proc, Jinzhong 030619, Peoples R China
基金
中国国家自然科学基金;
关键词
Pinelliae Rhizoma Praeparatum Cum Alumine; Polysaccharides; Gut microbiota; HUMAN INTESTINAL MICROBIOTA; OBESITY; SALIVA; ACID; MECHANISMS; EXTRACTION; TERNATA;
D O I
10.1016/j.ijbiomac.2024.131391
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Polysaccharides from Pinelliae Rhizoma Praeparatum Cum Alumine (PPA) have various biological activities, but their properties after oral administration are not clear. In this study, the absorption, digestion and fermentation properties of PPA were studied using in vivo fluorescence tracking, in vitro simulated digestion and fecal fermentation experiments. The absorption experiment showed that fluorescence was only observed in the gastrointestinal system, indicating that PPA could not be absorbed. Simulated digestion results showed that there were no significant changes in the molecular weight, Fourier transform infrared spectroscopy (FT-IR) spectrum, monosaccharides and reducing sugar of PPA during the digestion process, showing that the overall structure of PPA was not damaged. However, the carbohydrate gel electrophoresis bands of PPA enzymatic hydrolysates after simulated digestion were significantly changed, indicating that simulated digestion might impact the configuration of PPA. In vitro fermentation showed that PPA could be degraded by microorganisms to produce short chain fatty acids, leading to a decrease in pH value. PPA can promote the proliferation of Bacteroideaceae, Megasphaera, Bacteroideaceae, and Bifidobacteriaceae, and inhibit the growth of Desulfobacteriota and Enterobacteriaceae. The results indicated that PPA could treat diseases by regulating gut microbiota, providing a scientific basis for the application and development of PPA.
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页数:14
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