A Detailed Protocol for the Induction of Anemia and RBC Transfusion- associated Necrotizing Enterocolitis in Neonatal Mice

被引：1

作者：

Ramatchandirin, Balamurugan ^{[1
]}

Balamurugan, Marie Amalie ^{[1
]}

Desiraju, Suneetha ^{[2
]}

Chung, Yerin ^{[2
]}

Mohankumar, Krishnan ^{[1
,3
,4
]}

机构：

[1] Univ Nebraska Med Ctr, Dept Biochem & Mol Biol, Omaha, NE 68198 USA

[2] Johns Hopkins Univ, Sch Med, Dept Pediat, Baltimore, MD USA

[3] Univ Nebraska Med Ctr, Childrens Hlth & Res Inst, Omaha, NE 68198 USA

[4] Univ Nebraska Med Ctr, Dept Pediat, Omaha, NE 68198 USA

来源：

BIO-PROTOCOL | 2024年 / 14卷 / 10期

基金：

美国国家卫生研究院;

关键词：

Phlebotomy-induced anemia; RBC transfusion; Necrotizing enterocolitis; Neonatal mice; Intestinal injury; BLOOD-CELL TRANSFUSIONS; INFANTS;

D O I：

10.21769/BioProtoc.4993

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Anemia is a common and serious health problem, nearly universally diagnosed in preterm infants, and is associated with increased morbidity and mortality worldwide. Red blood cell (RBC) transfusion is a lifesaving and mainstay therapy; however, it has critical adverse effects. One consequence is necrotizing enterocolitis (NEC), an inflammatory bowel necrosis disease in preterm infants. The murine model of phlebotomy-induced anemia and RBC transfusion-associated NEC enables a detailed study of the molecular mechanisms underlying these morbidities and the evaluation of potential new therapeutic strategies. This protocol describes a detailed procedure for obtaining murine pups with phlebotomy-induced anemia and delivering an RBC transfusion that develops NEC.

引用

页数：11

共 24 条

[1] Refractory Pancytopenia and Megaloblastic Anemia due to Falciparum Malaria [J].

Aggarwal, Varun ;

Maheshwari, Anu ;

Rath, Bimbadhar ;

Kumar, Praveen ;

Basu, Srikanta .

JOURNAL OF TROPICAL PEDIATRICS, 2011, 57 (04) :283-285

[2] Adherence to NICU transfusion guidelines: data from a multihospital healthcare system [J].

Baer, V. L. ;

Lambert, D. K. ;

Schmutz, N. ;

Henry, E. ;

Stoddard, R. A. ;

Miner, C. ;

Wiedmeier, S. E. ;

Burnett, J. ;

Eggert, L. D. ;

Christensen, R. D. .

JOURNAL OF PERINATOLOGY, 2008, 28 (07) :492-497

[3] Necrotizing Enterocolitis in Infants With Congenital Heart Disease: The Role of Red Blood Cell Transfusions [J].

Baxi, Anand C. ;

Josephson, Cassandra D. ;

Iannucci, Glen J. ;

Mahle, William T. .

PEDIATRIC CARDIOLOGY, 2014, 35 (06) :1024-1029

[4] Necrotizing Enterocolitis: Long Term Complications [J].

Bazacliu, Catalina ;

Neu, Josef .

CURRENT PEDIATRIC REVIEWS, 2019, 15 (02) :115-124

[5] Pathophysiology of Necrotizing Enterocolitis: An Update [J].

Bazacliu, Catalina ;

Neu, Josef .

CURRENT PEDIATRIC REVIEWS, 2019, 15 (02) :68-87

[6] Is "transfusion-associated necrotizing enterocolitis" an authentic pathogenic entity? [J].

Christensen, Robert D. ;

Lambert, Diane K. ;

Henry, Erick ;

Wiedmeier, Susan E. ;

Snow, Gregory L. ;

Baer, Vickie L. ;

Gerday, Erick ;

Ilstrup, Sarah ;

Pysher, Theodore J. .

TRANSFUSION, 2010, 50 (05) :1106-1112

[7] Anemia of prematurity: how low is too low? [J].

Cibulskis, Catherine C. ;

Maheshwari, Akhil ;

Rao, Rakesh ;

Mathur, Amit M. .

JOURNAL OF PERINATOLOGY, 2021, 41 (06) :1244-1257

[8] Red blood cell transfusion in premature infants leads to worse necrotizing enterocolitis outcomes [J].

Cunningham, Kellie E. ;

Okolo, Frances C. ;

Baker, Robyn ;

Mollen, Kevin P. ;

Good, Misty .

JOURNAL OF SURGICAL RESEARCH, 2017, 213 :158-165

[9] A consistent approach to platelet transfusion in the NICU [J].

Del Vecchio, Antonio ;

Motta, Mario ;

Radicioni, Maurizio ;

Christensen, Robert D. .

JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE, 2012, 25 :93-96

[10] Effect of birth weight on the association between necrotising enterocolitis and red blood cell transfusions in ≤1500 g infants [J].

Elabiad, Mohamad Tammam ;

Harsono, Mimily ;

Talati, Ajay J. ;

Dhanireddy, Ramasubbareddy .

BMJ OPEN, 2013, 3 (11)

← 1 2 3 →