The Effect of Thermosonication Treatment on the Inactivation of Clostridium perfringens Spores

被引：0
作者：
Liang, Dong [1 ,2 ]
Cui, Xiaoshuang [1 ,2 ,3 ]
Guo, Shiliang [4 ]
Liu, Shijie [1 ,2 ]
Li, Miaoyun [1 ,2 ]
He, Lijun [3 ]
Zhao, Gaiming [1 ,2 ]
Zhu, Yaodi [1 ,2 ]
Zhao, Lijun [1 ,2 ]
Ma, Yangyang [1 ,2 ]
机构：
[1] Henan Agr Univ, Coll Food Sci & Technol, Int Joint Lab Meat Proc & Safety Henan Prov, 63 Wenhua Rd, Zhengzhou 450002, Peoples R China
[2] Henan Agr Univ, Int Joint Lab Meat Proc & Safety Henan Prov, 63 Wenhua Rd, Zhengzhou 450002, Peoples R China
[3] Henan Univ Technol, Coll Food Sci & Technol, Zhengzhou 450001, Peoples R China
[4] Henan Shuanghui Investment & Dev Co Ltd, Luohe 462000, Peoples R China
来源：
FOOD AND BIOPROCESS TECHNOLOGY | 2024年
关键词：
Ultrasound; Thermosonication; Clostridium perfringens spores; Inactivation mechanism; ULTRASOUND TREATMENTS; GERMINATION; PROTEINS; HEAT; FOOD;
D O I：
10.1007/s11947-024-03466-1
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Clostridium perfringens spores, which are extremely resistant and difficult to kill, can easily cause food spoilage and foodborne diseases. In this study, the effect of simultaneous ultrasound and heat (thermosonication, TS) treatment for C. perfringens spore inactivation was investigated. The TS treatment at 26.67 W/mL and 80 degrees C or 90 degrees C for 30 min resulted in 5.77 and 5.89 log inactivation of C. perfringens spores, respectively. A strong synergistic effect of TS treatment on spore inactivation was observed. The mechanism of TS treatment on spore inactivation was further investigated by comparing the structural changes and release of substances in the spore core using microscopic and spectroscopic assays. The results revealed that TS treatment directly inactivated C. perfringens spores by destroying spore coat, cortex, and inner membrane, along with the release of calcium dipicolinic acid (Ca2+-DPA), protein, nucleic acid, and other substances in the spore core. This study could provide a method to control C. perfringens spores in food industry.
引用
收藏
页码：349 / 359
页数：11
相关论文
共 42 条
  • [31] Infrared and confocal Raman spectroscopy to differentiate changes in the protein secondary structure in normal and abnormal thyroid tissues
    Soto, Claudio A. Tellez
    Medeiros-Neto, Lazaro P.
    dos Santos, Laurita
    Santos, Andre B. O.
    Ferreira, Isabelle
    Singh, Priyanka
    Canevari, Renata A.
    Martin, Airton A.
    [J]. JOURNAL OF RAMAN SPECTROSCOPY, 2018, 49 (07) : 1165 - 1173
  • [32] Characterization of Putative Sporulation and Germination Genes in Clostridium perfringens Food-Poisoning Strain SM101
    Talukdar, Prabhat K.
    Sarker, Mahfuzur R.
    [J]. MICROORGANISMS, 2022, 10 (08)
  • [33] The serine proteases CspA and CspC are essential for germination of spores of Clostridium perfringens SM101 through activating SleC and cortex hydrolysis
    Talukdar, Prabhat K.
    Sarker, Mahfuzur R.
    [J]. FOOD MICROBIOLOGY, 2020, 86
  • [34] Modelling Alicyclobacillus acidoterrestris inactivation in apple juice using thermosonication treatments
    Tremarin, Andreia
    Canbaz, Emine Asik
    Brandao, Teresa R. S.
    Silva, Cristina L. M.
    [J]. LWT-FOOD SCIENCE AND TECHNOLOGY, 2019, 102 : 159 - 163
  • [35] Application of ultraviolet radiation and ultrasound treatments for Alicyclobacillus acidoterrestris spores inactivation in apple juice
    Tremarin, Andreia
    Brandao, Teresa R. S.
    Silva, Cristina L. M.
    [J]. LWT-FOOD SCIENCE AND TECHNOLOGY, 2017, 78 : 138 - 142
  • [36] The effect of disulfide bonds on protein folding, unfolding, and misfolding investigated by FT-Raman spectroscopy
    Wang, Chih-Hsien
    Huang, Chia-Chi
    Lin, Long-Liu
    Chen, Wenlung
    [J]. JOURNAL OF RAMAN SPECTROSCOPY, 2016, 47 (08) : 940 - 947
  • [37] Effects of wet heat treatment on the germination of individual spores of Clostridium perfringens
    Wang, G.
    Paredes-Sabja, D.
    Sarker, M. R.
    Green, C.
    Setlow, P.
    Li, Y-q.
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2012, 113 (04) : 824 - 836
  • [38] Comparative real-time analysis of Saccharomyces cerevisiae cell viability, injury and death induced by ultrasound (20 kHz) and heat for the application of hurdle technology
    Wordon, B. A.
    Mortimer, B.
    McMaster, L. D.
    [J]. FOOD RESEARCH INTERNATIONAL, 2012, 47 (02) : 134 - 139
  • [39] Studies of the Commitment Step in the Germination of Spores of Bacillus Species
    Yi, Xuan
    Setlow, Peter
    [J]. JOURNAL OF BACTERIOLOGY, 2010, 192 (13) : 3424 - 3433
  • [40] Preparation of an AgNPs@Polydimethylsiloxane (PDMS) multi-hole filter membrane chip for the rapid identification of food-borne pathogens by surface-enhanced Raman spectroscopy
    Zhu, Yaodi
    Liu, Shijie
    Li, Miaoyun
    Liu, Weijia
    Wei, Zhanyong
    Zhao, Lijun
    Liu, Yanxia
    Xu, Lina
    Zhao, Gaiming
    Ma, Yangyang
    [J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2022, 267
12345