Nanopore Blockade Sensors for Quantitative Analysis Using an Optical Nanopore Assay

被引：1

作者：

Doan, Thanh Hoang Phuong ^{[1
]}

Fried, Jasper P. ^{[1
]}

Tang, Wenxian ^{[1
]}

Hagness, Daniel Everett ^{[1
]}

Yang, Ying ^{[1
]}

Wu, Yanfang ^{[1
]}

Tilley, Richard D. ^{[1
,2
]}

Gooding, J. Justin ^{[1
]}

机构：

[1] Univ New South Wales, Australian Ctr NanoMed, Sch Chem, Sydney, NSW 2052, Australia

[2] Univ New South Wales, Mark Wainwright Analyt Ctr, Electron Microscope Unit, Sydney, NSW 2052, Australia

来源：

NANO LETTERS | 2024年 / 24卷 / 21期

基金：

英国医学研究理事会; 澳大利亚研究理事会;

关键词：

Solid-state nanopores; fluorescence; single-moleculesensing; aptamers; optical sensing; SINGLE-MOLECULE DETECTION; PROTEINS;

D O I：

10.1021/acs.nanolett.4c00530

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nanopore sensing is a popular biosensing strategy that is being explored for the quantitative analysis of biomarkers. With low concentrations of analytes, nanopore sensors face challenges related to slow response times and selectivity. Here, we demonstrate an approach to rapidly detect species at ultralow concentrations using an optical nanopore blockade sensor for quantitative detection of the protein vascular endothelial growth factor (VEGF). This sensor relies on monitoring fluorescent polystyrene nanoparticles blocking nanopores in a nanopore array of 676 nanopores. The fluorescent signal is read out using a wide-field fluorescence microscope. Nonspecific blockade events are then distinguished from specific blockade events based on the ability to pull the particles out of the pore using an applied electric field. This allows the detection of VEGF at sub-picomolar concentration in less than 15 min.

引用

页码：6218 / 6224

页数：7

共 26 条

[1] Small molecule electro-optical binding assay using nanopores
Cai, Shenglin
Sze, Jasmine Y. Y.
Ivanov, Aleksandar P.
Edel, Joshua B.
[J]. NATURE COMMUNICATIONS, 2019, 10 (1)
[2] Single molecule detection; from microscopy to sensors
Chauhan, Nidhi
Saxena, Kirti
Jain, Utkarsh
[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2022, 209 : 1389 - 1401
[3] Nanopore blockade sensors for ultrasensitive detection of proteins in complex biological samples
Chuah, Kyloon
Wu, Yanfang
Vivekchand, S. R. C.
Gaus, Katharina
Reece, Peter J.
Micolich, Adam P.
Gooding, J. Justin
[J]. NATURE COMMUNICATIONS, 2019, 10 (1)
[4] Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays
Farka, Zdenek
Mickert, Matthias J.
Pastucha, Matej
Mikusova, Zuzana
Skladal, Petr
Gorris, Hans H.
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2020, 59 (27) : 10746 - 10773
[5] Optical Nanopore Sensors for Quantitative Analysis
Fried, Jasper P.
Wu, Yanfang
Tilley, Richard D.
Gooding, J. Justin
[J]. NANO LETTERS, 2022, 22 (03) : 869 - 880
[6] In situ solid-state nanopore fabrication
Fried, Jasper P.
Swett, Jacob L.
Nadappuram, Binoy Paulose
Mol, Jan A.
Edel, Joshua B.
Ivanov, Aleksandar P.
Yates, James R.
[J]. CHEMICAL SOCIETY REVIEWS, 2021, 50 (08) : 4974 - 4992
[7] Single-Molecule Sensors: Challenges and Opportunities for Quantitative Analysis
Gooding, J. Justin
Gaus, Katharina
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2016, 55 (38) : 11354 - 11366
[8] DNA capture into a nanopore: Interplay of diffusion and electrohydrodynamics
Grosberg, Alexander Y.
Rabin, Yitzhak
[J]. JOURNAL OF CHEMICAL PHYSICS, 2010, 133 (16)
[9] Stochastic sensing of organic analytes by a pore-forming protein containing a molecular adapter
Gu, LQ
Braha, O
Conlan, S
Cheley, S
Bayley, H
[J]. NATURE, 1999, 398 (6729) : 686 - 690
[10] Nanosensors lost in space. A random walk study of single molecule detection with single-nanopore sensors
Hoefler, Lajos
Gyurcsanyi, Robert E.
[J]. ANALYTICA CHIMICA ACTA, 2012, 722 : 119 - 126

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