Assessment of Circulating Cell Free DNA Methylation Biomarker of Panel of Selected Genes for Lung Cancer Detection

被引：0
作者：
Karpathak, Supriya [1 ,2 ,3 ]
Garg, Rajiv [1 ]
Ahmad, Mohammad Kaleem [2 ]
Srivastava, Anand [1 ]
Qidwai, Tabish [3 ]
Ubaid, Saba [2 ]
Srivastav, Anurag Kumar [4 ]
机构：
[1] King Georges Med Univ, Dept Resp Med, Shah Mina Rd Chowk, Lucknow 226003, UP, India
[2] King Georges Med Univ, Dept Biochem, Shah Mina Rd Chowk, Lucknow 226003, UP, India
[3] Shri Ramswaroop Mem Univ, Inst Biosci & Technol, Lucknow Deva Rd, Barabanki 225003, UP, India
[4] SGPGIMS, Dept Clin Immunol & Rheumatol, Lucknow 226014, UP, India
来源：
INDIAN JOURNAL OF CLINICAL BIOCHEMISTRY | 2024年
关键词：
Lung cancer; Methylation; Gene expression; TSGs; Biomarker; PROMOTER-HYPERMETHYLATION; TUMOR-SUPPRESSOR; E-CADHERIN; CAVEOLIN-1; EXPRESSION; CHFR; CARCINOMA; GROWTH; CDH1; METASTASIS;
D O I：
10.1007/s12291-024-01255-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Lung cancer (LC) is a well-known cause of cancer-related death worldwide. Early diagnosis of LC is an unmet need to improve the treatment response. In present study, we have analyzed the promoter methylation of a panel of genes in LC tissue and established tissue mirror biomarkers in plasma-derived circulating cell-free DNA (cfDNA). The mRNA expression of panel of genes (CDH1, CHFR, SOX10, and CAV1) were analyzed in tumor tissue (TT) including Non-Small Cell Lung Cancer (NSCLC), and Small Cell Lung Cancer (SCLC) as compared to tumor-free tissue (TF) and correlated with gene promoter methylation in tissue-derived gDNA using methylation-specific PCR. The methylation analysis of genes were further analyzed in plasma-derived cfDNA of NSCLC and SCLC compared to healthy control. The methylation frequencies of CDH1, CHFR, and SOX10 were significantly higher (p < 0.05 to p < 0.001) in TT (59.45, 45.94 and 72.97%) compared to TF (21.42, 14.28 to 28.57%) respectively. Gene expressions in TT were down-regulated (p < 0.01) in contrast to TF and negatively correlated (p < 0.001) with hypermethylation, except CAV1, which showed up-regulation with hypomethylation in TT of NSCLC. The methylation status in cfDNA exploits similar results as tissue sample, exhibited significant hypermethylation (p <= 0.05 to p <= 0.001) in cfDNA of LC as compared to healthy control, and showed 58.33 to 76.47% positive predictive value and 51.47 to 66.33% negative predictive value. This study illustrated that gene promoter methylation analysis of the panel of genes in plasma samples has the potential for diagnosis of LC, and can be used as a tissue mirror biomarker.
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页数：15
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共 60 条
[41]   Reduction of caveolin 1 gene expression in lung carcinoma cell lines [J].
Racine, C ;
Bélanger, M ;
Hirabayashi, H ;
Boucher, M ;
Chakir, J ;
Couet, J .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 255 (03) :580-586
[42]   Caveolin-1 expression is down-regulated in cells transformed by the human papilloma virus in a p53-dependent manner. Replacement of caveolin-1 expression suppresses HPV-mediated cell transformation [J].
Razani, B ;
Altschuler, Y ;
Zhu, L ;
Pestell, RG ;
Mostov, KE ;
Lisanti, MP .
BIOCHEMISTRY, 2000, 39 (45) :13916-13924
[43]   First-line therapy and methylation status of CHFR in serum influence outcome to chemotherapy versus EGFR tyrosine kinase inhibitors as second-line therapy in stage IV non-small-cell lung cancer patients [J].
Salazar, Fernanda ;
Angel Molina, Miguel ;
Sanchez-Ronco, Maria ;
Moran, Teresa ;
Luis Ramirez, Jose ;
Miguel Sanchez, Jose ;
Stahel, Rolf ;
Garrido, Pilar ;
Cobo, Manuel ;
Isla, Dolores ;
Bertran-Alamilloh, Jordi ;
Massuti, Bartomeu ;
Cardenal, Felipe ;
Manegold, Christian ;
Lianes, Pilar ;
Manuel Trig, Jose ;
Javier Sanchez, Jose ;
Taron, Miquel ;
Rosell, Rafael .
LUNG CANCER, 2011, 72 (01) :84-91
[44]   Multifaceted Roles of Caveolin-1 in Lung Cancer: A New Investigation Focused on Tumor Occurrence, Development and Therapy [J].
Shi, Yu-Bo ;
Li, Jun ;
Lai, Xing-Ning ;
Jiang, Rui ;
Zhao, Rui-Chen ;
Xiong, Li-Xia .
CANCERS, 2020, 12 (02)
[45]   Chfr expression is downregulated by CpG island hypermethylation in esophageal cancer [J].
Shibata, Y ;
Haruki, N ;
Kuwabara, Y ;
Ishiguro, H ;
Shinoda, N ;
Sato, A ;
Kimura, M ;
Koyama, H ;
Toyama, T ;
Nishiwaki, T ;
Kudo, J ;
Terashita, Y ;
Konishi, S ;
Sugiura, H ;
Fujii, Y .
CARCINOGENESIS, 2002, 23 (10) :1695-1699
[46]   Methylation pattern of caveolin-1 in prostate cancer as potential cfDNA biomarker [J].
Skara, Lucija ;
Vodopic, Tonei ;
Pezelj, Ivan ;
Abramovie, Irena ;
Vrhovec, Borna ;
Vrtarie, Alen ;
Sincic, Nino ;
Tomas, Davor ;
Bulimbasic, Stela ;
Kulis, Tomislav ;
Ulamec, Monika .
BIOMOLECULES AND BIOMEDICINE, 2023, 23 (01) :176-186
[47]   Caveolin-1 inhibits breast cancer growth and metastasis [J].
Sloan, EK ;
Stanley, KL ;
Anderson, RL .
ONCOGENE, 2004, 23 (47) :7893-7897
[48]   Quantification of free circulating DNA as a diagnostic marker in lung cancer [J].
Sozzi, G ;
Conte, D ;
Leon, ME ;
Cirincione, R ;
Roz, L ;
Ratcliffe, C ;
Roz, E ;
Cirenei, N ;
Bellomi, M ;
Pelosi, G ;
Pierotti, MA ;
Pastorino, U .
JOURNAL OF CLINICAL ONCOLOGY, 2003, 21 (21) :3902-3908
[49]   Different roles for caveolin-1 in the development of non-small cell lung cancer versus small cell lung cancer [J].
Sunaga, N ;
Miyajima, K ;
Suzuki, M ;
Sato, M ;
White, MA ;
Ramirez, RD ;
Shay, JW ;
Gazdar, AF ;
Minna, JD .
CANCER RESEARCH, 2004, 64 (12) :4277-4285
[50]   Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J].
Sung, Hyuna ;
Ferlay, Jacques ;
Siegel, Rebecca L. ;
Laversanne, Mathieu ;
Soerjomataram, Isabelle ;
Jemal, Ahmedin ;
Bray, Freddie .
CA-A CANCER JOURNAL FOR CLINICIANS, 2021, 71 (03) :209-249
123456