Ginsenoside Rg1 Induces Autophagy in Colorectal Cancer through Inhibition of the Akt/mTOR/p70S6K Pathway

被引:5
作者
Liu, Ruiqi [1 ]
Zhang, Bin [2 ]
Zou, Shuting [1 ]
Cui, Li [1 ]
Lin, Lin [3 ]
Li, Lingchang [1 ]
机构
[1] Jiangsu Prov Acad Tradit Chinese Med, Nanjing 210028, Jiangsu, Peoples R China
[2] Nanjing Lishui Dist Hosp Tradit Chinese Med, Nanjing 211200, Jiangsu, Peoples R China
[3] Guangzhou Univ Chinese Med, Shenzhen Hosp, Gastroenterol, Shenzhen 518000, Guangdong, Peoples R China
关键词
Ginsenoside Rg1; autophagy; colon cancer; CELL-DEATH; PI3K/AKT/MTOR PATHWAY; APOPTOSIS; ENHANCEMENT; ACTIVATION; GROWTH;
D O I
10.4014/jmb.2310.10043
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This study aimed to elucidate the anti -colon cancer mechanism of ginsenoside Rg1 in vitro and in vivo. Cell viability rate was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tetrazolium assay. The inhibitory effect of ginsenoside Rg1 against CT26 cell proliferation gradually increased with increasing concentration. The in vivo experiments also demonstrated an antitumor effect. The monodansylcadaverine (MDC), transmission electron microscopy (TEM), and expression of autophagy marker proteins confirmed that ginsenoside Rg1 induced autophagy in vitro. Ginsenoside Rg1 induced autophagy death of CT26 cells, but this effect could be diminished by autophagy inhibitor (3-methyladenine, 3 -MA). Additionally, in a xenograft model, immunohistochemical analysis of tumor tissues showed that the LC3 and Beclin-1 proteins were highly expressed in the tumors from the ginsenoside Rg1-treated nude mice, confirming that ginsenoside Rg1 also induced autophagy in vivo. Furthermoer, both in vivo and in vitro, the protein expressions of p-Akt, p-mTOR, and p-p70S6K were inhibited by ginsenoside Rg1, which was verified by Akt inhibitors. These results indicated that the mechanism of ginsenoside Rg1 against colon cancer was associated with autophagy through inhibition of the Akt/mTOR/p70S6K signaling pathway.
引用
收藏
页码:774 / 782
页数:9
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