Whole-Genome Sequence Analysis of Antibiotic Resistance, Virulence, and Plasmid Dynamics in Multidrug-Resistant E. coli Isolates from Imported Shrimp

被引:3
作者
Sung, Kidon [1 ]
Nawaz, Mohamed [1 ]
Park, Miseon [1 ]
Chon, Jungwhan [2 ]
Khan, Saeed A. [1 ]
Alotaibi, Khulud [3 ]
Revollo, Javier [4 ]
Miranda, Jaime A. [4 ]
Khan, Ashraf A. [1 ]
机构
[1] US FDA, Div Microbiol, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA
[2] Inje Univ, Dept Compan Anim Hlth, Gimhae 50834, South Korea
[3] Saudi Food & Drug Author SFDA, Riyadh 13513, Saudi Arabia
[4] US FDA, Div Genet & Mol Toxicol, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA
关键词
whole-genome sequence; E; coli; imported shrimp; ESCHERICHIA-COLI; ANTIMICROBIAL RESISTANCE; SULFONAMIDE RESISTANCE; TRANSMISSION; RESIDUES; BACTERIA; DATABASE; STATES; GENES; FISH;
D O I
10.3390/foods13111766
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
We analyzed antimicrobial resistance and virulence traits in multidrug-resistant (MDR) E. coli isolates obtained from imported shrimp using whole-genome sequences (WGSs). Antibiotic resistance profiles were determined phenotypically. WGSs identified key characteristics, including their multilocus sequence type (MLST), serotype, virulence factors, antibiotic resistance genes, and mobile elements. Most of the isolates exhibited resistance to gentamicin, streptomycin, ampicillin, chloramphenicol, nalidixic acid, ciprofloxacin, tetracycline, and trimethoprim/sulfamethoxazole. Multilocus sequence type (MLST), serotype, average nucleotide identity (ANI), and pangenome analysis showed high genomic similarity among isolates, except for EC15 and ECV01. The EC119 plasmid contained a variety of efflux pump genes, including those encoding the acid resistance transcriptional activators (gadE, gadW, and gadX), resistance-nodulation-division-type efflux pumps (mdtE and mdtF), and a metabolite, H1 symporter (MHS) family major facilitator superfamily transporter (MNZ41_23075). Virulence genes displayed diversity, particularly EC15, whose plasmids carried genes for adherence (faeA and faeC-I), invasion (ipaH and virB), and capsule (caf1A and caf1M). This comprehensive analysis illuminates antimicrobial resistance, virulence, and plasmid dynamics in E. coli from imported shrimp and has profound implications for public health, emphasizing the need for continued surveillance and research into the evolution of these important bacterial pathogens.
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页数:23
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