Ovine papillomavirus type 3 virus-like particle-based tools for diagnosis and detection of infection

被引：0

作者：

Cacciotto, Carla ^{[1
,2
]}

Dore, Gian Mario ^{[1
]}

Cubeddu, Tiziana ^{[1
,2
]}

Burrai, Giovanni Pietro ^{[1
,2
]}

Anfossi, Antonio Giovanni ^{[1
]}

Antuofermo, Elisabetta ^{[1
,2
]}

Varoni, Maria Vittoria ^{[1
]}

Demontis, Maria Piera ^{[1
]}

Zobba, Rosanna ^{[1
]}

Pittau, Marco ^{[1
,2
]}

Mueller, Martin ^{[3
]}

Alberti, Alberto ^{[1
,2
]}

机构：

[1] Univ Sassari, Dipartimento Med Vet, 2 Via Vienna, I-07100 Sassari, Italy

[2] Mediterranean Ctr Dis Control, Sassari, Italy

[3] German Canc Res Ctr, Heidelberg, Germany

来源：

VACCINE | 2024年 / 42卷 / 22期

关键词：

Animal papillomavirus; Skin cancer; Oncovirus; Vaccines; Serogical diagnosis; PSEUDO-SARCOID FORMATION; PROTEIN; VACCINE; DNA; IMMUNOGENICITY; PURIFICATION; IMMUNIZATION; PREVENTION; RABBITS; SYSTEM;

D O I：

10.1016/j.vaccine.2024.06.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The design of prophylactic and diagnostic tools specific to animal papillomaviruses is hampered by the difficulties of viral in vitro manipulation and by the scarce availability of dedicated biotechnological tools. This paper reports the production of Ovine Papillomavirus 3 (OaPV3)-based virus-like particles (OaPV3-VLPs) in the baculovirus system and their use to investigate host humoral immune response through the establishment of an indirect ELISA test., Polyclonal sera and monoclonal antibodies were generated against OaPV3-VLPs, and their isotype and reactivity were determined. Additionally, antibodies allowed OaPV3 detection in ovine squamous cell carcinoma (SCC) samples by immunohistochemistry. Results encourage the standardization of OaPV3specific prophylactic and serological diagnostic tools, and open new perspectives for the study of host-viral interaction and SCC development.

引用

页数：11

共 56 条

[1] Ovis aries Papillomavirus 3: A prototype of a novel genus in the family Papillomaviridae associated with ovine squamous cell carcinoma [J].